This book presents latest work in the field of plant biotechnology regarding high-efficiency micropropagation for commercial exploitation at low labor and equipment costs. The book consists of 18 chapters on establishing advanced culture systems, techniques as well as latest modification protocols on a variety of crops. It also discusses new methods such as nylon film culture system, light-emitting diode and wireless light-emitting diode system, stem elongation, wounding manipulation and shoot tip removal, in vitro hydroponic and microponic culture system, thin cell layer culture system etc. Plant cell tissue has been developed more than fifty years ago. Since then applications of in vitro plant propagation expanded rapidly all around the world and played as an important role in agricultural and horticultural systems.

Chapter 1. General information: some aspects of plant tissue culture.- Chapter 2. The use of the paraffin embedding method in the study of cultured explants I: Background information.- Chapter 3. The paraffin embedding method II: Protocols.- Chapter 4. A simple guide to the use of compresstome in plant research.- Chapter 5. Establishment of nylon bag culture system in regeneration and micropropagation.- Chapter 6. Wounding manipulation and shoot tip removal methods in the micropropagation of Paphiopedilum callosum.- Chapter 7. Stem elongation for plant micropropagation.- Chapter 8. Somatic embryogenesis as a tool for propagating of some plants.- Chapter 9. Light-Emitting Diodes (LEDs) in plant regeneration, growth and secondary metabolite accumulation.- Chapter 10. In vitro hydroponic culture system in plant micropropagation.- Chapter 11. Microponic culture system in the propagation of some plants.- Chapter 12. The application of thin cell layers culture technique in plant regeneration and micropropagation - latest achievements.- Chapter 13. In vitro flowering of T. fournieri.- Chapter 14. The use of silver nanoparticles as a disinfectant and media additive in plant micropropagation.- Chapter 15. Enhanced growth and overcoming abnormal phenomena in micropropagation by nanoparticles.- Chapter 16. A protocol of shoot regeneratin and polyploid plantlet production in P. villosum.- Chapter 17. In vitro growth and development of plants under stimulated microgravity condition.- Chapter 18. Wireless light-emitting diode system for micropropagating of Chrysanthemum and Strawberry.