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Transcriptome Analysis
ISBN-13:
9788876426421
Veröffentl:
2018
Seiten:
188
Autor:
Alessandro Cellerino
Serie:
17, Publications of the Scuola Normale Superiore Lecture Notes (Scuola Normale Superiore)
eBook Typ:
PDF
eBook Format:
EPUB
Kopierschutz:
1 - PDF Watermark
Sprache:
Englisch
Beschreibung:
The goal of this book is to be an accessible guide for undergraduate and graduate students to the new field of data-driven biology. Next-generation sequencing technologies have put genome-scale analysis of gene expression into the standard toolbox of experimental biologists. Yet, biological interpretation of high-dimensional data is made difficult by the lack of a common language between experimental and data scientists. By combining theory with practical examples of how specific tools were used to obtain novel insights in biology, particularly in the neurosciences, the book intends to teach students how to design, analyse, and extract biological knowledge from transcriptome sequencing experiments.
Preface.- Introduction: why study transcriptomics?.- 1. Data distribution and visualisation.- 2. Next-generation RNA sequencing.- 3. RNA-seq raw data processing.- 4. Differentially expressed gene detection & analysis.- 5. Unbiased clustering methods.- 6. Knowledge-based clustering methods.- 7. Network analysis.- 8. Mesoscale transcriptome analysis.- 9. Microscale transcriptome analysis.- Bibliography.- Index.
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