Swamp eels have been widely accepted as sources of food, especially among various Asian cultures. However, their potential values as novel sources of therapeutic agents have not been widely appreciated. Like most other tropical fishes and amphibians, the outer integumentary system of Monopterus albus is covered with mucus layers, which may act as mechanical and biochemical barrier for their skin. The biochemical components of these mucus layers may have certain compounds that may be medically beneficial to human. The current study was interested to screen of the eel skin mucus extracts for bioactive compounds using gas chromatography-mass spectrometry and liquid chromatography quadrupole-time-of-flight mass spectrometry. Several compounds, which had been previously identified to have different biological activities mainly cytotoxic, antioxidant and antimicrobial activities were discovered.