Steering clear of quantum mechanics and product operators, "Pocket Guide to Biomolecular NMR" uses intuitive, concrete analogies to explain the theory required to understand NMR studies on the structure and dynamics of biological macromolecules. For example, instead of explaining nuclear spin with angular momentum equations or Hamiltonians, the books describes nuclei as "bells" in a choir, ringing at specific frequencies depending on the atom type and their surrounding electromagnetic environment.This simple bell analogy, which is employed throughout the book, has never been used to explain NMR and makes it surprisingly easy to learn complex, bewildering NMR concepts, such as dipole-dipole coupling and CPMG pulse sequences. Other topics covered include the basics of multi-dimensional NMR, relaxation theory, and Model Free analysis. The small size and fast pace of ¿Pocket Guide to Biomolecular NMR¿ makes the book a perfect companion to traditional biophysics and biochemistry textbooks, but the book's unique perspective will provide even seasoned spectroscopists with new insights and handy ¿thought¿ short-cuts.

Easy-to-understand guide into the subject

1 Atomic Bells and Frequency Finders1.1 Chemical Choirs1.2 Essentials of Electromagnetism1.3 Electromagnetic Microsensors1.4 Frequency FindersMathematical Sidebar 1.1: Fourier Transform1.5 Basics of one-dimensional NMRMathematical Sidebar 1.2 Converting Hz to PPMReferences2 Bonded Bells and Two-Dimensional Spectra2.1 Introduction to Coupling2.2 Bonded Bells: J-CouplingMathematical Sidebar 2.1: Karplus Equation2.3 NMR Maps: Two-Dimensional SpectraMathematical Sidebar 2.2 Why 12C and 14N atoms are so shy?2.4 The 1H-15N HSQC: Our Bread and Butter2.5 Hidden Notes: Creating Two-Dimensional SpectraReferences3 Neighboring Bells and Structure Bundles3.1 Bumping Bells: Dipole-Dipole CouplingMathematical Sidebar 3.1: Dipole-dipole Coupling3.2 Atomic Meter Stick: the NOE3.3 Into "Three-D"3.4 Adult "Connect-the-Dots:" HNCA3.5 Putting the Pieces Together: A Quick Review3.6 Wet Noodles and Proteins Bundles: Building a Three-Dimensional StructureReferences4 Relaxation Theory Part One: Silencing of the Bells4.1 Nothing Rings Forever: Two Paths to Relax4.2 Relaxation: Ticket to the Protein PromMathematical Sidebar 4.1: Boltzmann Distribution4.3 Oh-My, How Your Field Fluctuates4.4 Blowing Off Steam and Returning to Equilibrium: T1Mathematical Sidebar 4.2: T1 Relaxation4.5 Loosing Lock-Step : Coherence and T2Mathematical Sidebar 4.3: T2 Relaxation and Spin EchoReferences5 Relaxation Theory Part Two: Moving Atoms and Changing Notes5.1 Keeping the Terms Straight5.2 NMR Dynamics in a Nutshell: The Rules of Exchange5.3 Two States, One Peak: Atoms in the Fast Lane of Exchange5.4 Two States, Two Peaks: Atoms in the Slow Lane of Exchange5.5 Two States, One Strange Peak: Atoms in Intermediate Exchange5.6 Tumbling Together: Rotational Correlation Time (¿c)5.7 SummaryReferences6 Protein Dynamics6.1 Dynamics Analysis by NMR: Multli-Channel Metronomes, Not a GPS6.2 Elegant Simplicity: Lipari and Szabo Throw Out the Models6.3 Wagging Tails and Wiggling Bottoms: Local versus Global Motion6.4 Measuring Fast Motion: Model Free AnalysisMathematical Sidebar 6.1: Correlation Functions and Model Free6.5 Changing Directions on the Track: Refocusing Pulses6.6 Measuring Intermediate Motion: CPMG Relaxation Dispersion Analysis6.7 Measuring Slow Motion: Z-Exchange Spectroscopy6.8 Measuring Motion SummaryReferences