Das wohl umfassendste Lehrbuch zu Methoden der modernen Biowissenschaften. Die physikalischen und chemischen Grundlagen sowie die Stärken und Schwächen jeder Methode werden detailliert erläutert.

Part I: PROTEIN ANALYTICSProtein purificationProtein determinationEnzymatic activity testsMicrocalorimetryImunological Techniques Cheminal modification of proteins and protein complexesSpectroscopyLight microscopy - ImagingCleavage of proteinsChromatographic techniquesElectrophoretic techniques Capillary electrophoresisAmino acid analysisProtein seqeunce analysisMass spectrometryProtein protein interactionsThe Two-Hybrid SystemTAP-Tagging and Purification of Protein ComplexesAnalyzing Interactions In Vitro: GST-PulldownSurface Plasmon Resonance Spectroscopy Fluorescence Resonance Energy Transfer (FRET)Analytical Ultracentrifugation Analytical Ultracentrifugation BiosensorsPart II: 3D STRUCTURE DETERMINATIONNMREPRElectron microscopyAtomic force microscopy X-ray structrure analysisPart III: PEPTIDES, CARBOHYDRATES, AND LIPIDSAnalysis of synthetic peptidesCarbohydrate analysisLipid analysisAnalysis of posttranslational modificationsPart IV: NUCLEIC ACID ANALYTICS Isolation and Purification of Nucleic AcidsAnalysis of Nucleic AcidsRestriction analysisElectrophoresisLC-MS of Oligonucleotides LC-MS of Oligonucleotides Techniques for the Hybridization and Detection of Nucleic AcidsPolymerase chain reaction DNA sequencingAnalysis of epigenetic modificationsProtein nucleic acid interactionsPart V: FUNCTIONAL AND SYSTEMS ANALYTICSSequence analysis by bioinformatics Analysis of promotor strengthHybridization of fluorescent DNA for genome analysis in molecular cytogeneticPhysical and genetic mapping of genomesDNA microarray techmology The Use of oligonucleotides as tools in cell biologyProteome analysisMetabolomics and peptidomicsInteractomics - systematic protein protein interactionsChemical BiologyToponome AnalysisMass Spectrometric Imaging