This monograph reviews all relevant technologies based on mass spectrometry that are used to study or screen biological interactions in general. Arranged in three parts, the text begins by reviewing techniques nowadays almost considered classical, such as affinity chromatography and ultrafiltration, as well as the latest techniques. The second part focusses on all MS-based methods for the study of interactions of proteins with all classes of biomolecules. Besides pull down-based approaches, this section also emphasizes the use of ion mobility MS, capture-compound approaches, chemical proteomics and interactomics. The third and final part discusses other important technologies frequently employed in interaction studies, such as biosensors and microarrays.For pharmaceutical, analytical, protein, environmental and biochemists, as well as those working in pharmaceutical and analytical laboratories.

PrefaceINTRODUCTION TO MASS SPECTROMETRY, A TUTORIALIntroductionFigures of MeritAnalyte IonizationMass Analyzer Building BlocksTandem Mass SpectrometryData Interpretation and Analytical StrategiesConclusion and PerspectivesPART I: Direct MS Based Affinity TechniquesSTUDYING PROTEIN-PROTEIN INTERACTIONS BY COMBINING NATIVE MASS SPECTROMETRY AND CHEMICAL CROSS-LINKINGIntroductionProtein Analysis by Mass SpectrometryNative MSChemical Cross-Linking MSValue of Combining Native MS with Chemical Cross-Linking MSRegulating the GiantCapturing Transient InteractionsAn Integrative Approach for Obtaining Low-Resolution Structures of Native Protein ComplexesFuture DirectionsNATIVE MASS SPECTROMETRY APPROACHES USING ION MOBILITY-MASS SPECTROMETRYIntroductionSample PreparationElectrospray IonizationMass Analyzers and Tandem MS ApproachesIon Mobility Data ProcessingChallenges and Future PerspectivesPART II: LC-MS Based with Indirect AssaysMETHODOLOGIES FOR EFFECT-DIRECTED ANALYSIS: ENVIRONMENTAL APPLICATIONS, FOOD ANALYSIS, AND DRUG DISCOVERYIntroductionPrinciple of Traditional Effect-Directed AnalysisSample PreparationFractionation for Bioassay TestingMiscellaneous ApproachesIdentification of Confirmation ProcessConclusion and PerspectivesMS BINDING ASSAYSIntroductionMS Binding Assays - StrategyApplication of MS Binding AssaysSummary and PerspectivesMETABOLIC PROFILING APPROACHES FOR THE IDENTIFICATION OF BIOACTIVE METABOLITES IN PLANTSIntroduction to Plant Metabolic ProfilingSample Collection and ProcessingHyphenated TechniquesMass SpectrometryMass Spectrometric ImagingData AnalysisFuture PerspectivesANTIVENOMICS: A PROTEOMICS TOOL FOR STUDYING THE IMMUNOREACTIVITY OF ANTIVENOMSIntroductionChallenge of Fighting Human Envenoming by SnakebitesToolbox for Studying the Immunological Profile of AntivenomsFirst-Generation AntivenomicsSnake VenomicsSecond-Generation AntivenomicsConcluding RemarksPART III: Direct Pre- and On-Column Coupled TechniquesFRONTAL AND ZONAL AFFINITY CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRYIntroductionFrontal Affinity ChromatographyStaircase MethodSimultaneous Frontal Analysis of a Complex MixtureMultiprotein Stationary PhaseZonal ChromatographyNonlinear ChromatographyONLINE AFFINITY ASSESSMENT AND IMMUNOAFFINITY SAMPLE PRETREATMENT IN CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRYIntroductionCapillary ElectrophoresisAffinity Capillary ElectrophoresisImmunoaffinity Capillary ElectrophoresisCapillary Electrophoresis-Mass SpectrometryApplication of ACE-MSApplications of IA-CE-MSConclusionsLABEL-FREE BIOSENSOR AFFINITY ANALYSIS COUPLED TO MASS SPECTROMETRYIntroduction to MS-Coupled Biosensor PlatformsStrategies for Coupling Label-Free Analysis with Mass SpectrometryNew Sensor and MS Platforms, Opportunities for IntegrationPART IV: Direct Post Column Coupled Affinity TechniquesHIGH-RESOLUTION SCREENING: POST-COLUMN CONTINUOUS-FLOW BIOASSAYSIntroductionThe High-Resolution Screening PlatformData AnalysisConclusions and PerspectivesCONCLUSIONSIndex