The first systematic overview of this key technique since the early 1990s, this authoritative reference is the only handbook available to include all recent developments. The author draws on his wide-ranging experience in both academia and industry to systematically cover all types of enzyme immobilization methods, such as adsorption-based and covalent immobilization, as well as enzyme entrapment and encapsulation. Throughout, a careful review of materials and techniques for the generation of functional immobilized enzymes benefits both developers and users of carrier-bound enzymes.A must for biotechnologists, biochemists and preparative chemists using enzymes in their daily work.

IMMOBILIZED ENZYMES: PAST, PRESENT AND PROSPECTSIntroductionThe PastImplications from the PastProspective and Future DevelopmentADSORPTION-BASED IMMOBILIZATIONIntroduction and ClassificationPrinciples of Adsorptive Enzyme ImmobilizationRequirements of the CarriersFactors which Dictate Enzyme PerformancePreparation of Immobilized Enzymes by AdsorptionCOVALENT ENZYME IMMOBILIZATIONIntroductionPhysical Nature of CarriersChemical Nature of CarriersAmino Acid Residues for Covalent Binding of EnzymesFactors Influencing Enzyme PerformancePreparation of Active CarriersENZYME ENTRAPMENTIntroduction and DefinitionRequirements of the CarriersEffect of the EntrapmentPreparation of Entrapped EnzymesENZYME ENCAPSULATIONIntroduction and ClassificationEffect of EncapsulationPreparation of Encapsulated EnzymesNON-CONVENTIONAL ENZYME IMMOBILIZATIONCoating-based ImmobilizationSite-specific ImmobilizationImmobilization in Organic SolventsImprinting ImmobilizationModification-based ImmobilizationPost-immobilization Techniques