This book offers readers cutting-edge research at the interface of polymer science and engineering, biomedical engineering, materials science, and biology. State-of-the-art developments in microscale technologies for cell engineering applications are covered, including technologies relevant to both pluripotent and adult stem cells, the immune system, and somatic cells of the animal and human origin.This book bridges the gap in the understanding of engineering biology at multiple length scale, including microenvironmental control, bioprocessing, and tissue engineering in the areas of cardiac, cartilage, skeletal, and vascular tissues, among others. This book also discusses unique, emerging areas of micropatterning and three-dimensional printing models of cellular engineering, and contributes to the better understanding of the role of biophysical factors in determining the cell fate. Microscale Technologies for Cell Engineering is valuable for bioengineers, biomaterial scientists, tissue engineers, clinicians, immunoengineers, immunologists and stem cell biologists, as it offers a review of the current cutting-edge cell engineering research at multiple length scale and will be valuable in developing new strategies for efficient scale-up and clinical translation.

Covers the stem cell manufacturing in labs for fundamental studies and transformative bioengineering applications in tissue engineering

Introduction.- Part I: Microscale Technologies for Controlling Stem Cell Microenvironments.- Microscale culture of pluripotent stem cells.- Microscale technology in cellular reprogramming.- Microfluidics-based technologies for stem cells and embryos.- Microfulidic approaches for screening Stem Cells from Blood.- Three dimensional culture models for developmental biology applications.- Biophysical control of hematopoietic stem and progenitor cells.- Influence of cellular mechanics on stem cell fate in 3D microenvironments.- Spatial patterning of multi-cellular stem cell aggregates.- Part II: Microscale Tissue Engineering using Stem Cells.- Spatially defined cell culture within three-dimensional hydrogels.- Combinatorial 3D Matrices for Human Embryonic Stem Cell Differentiation.- Engineered stem cell-based scaffolds and patches for heart disorders.- Engineered substrates for improved human pluripotent stem cell culture.- Stem cell expansion using microcarrier-based culture systems.- Neural differentiation of pluripotent stem cells in 3D microfibrous matrices.- Microscale approaches for molecular regulation during skeletal development.- Spatial Patterning of Stem Cells to Engineer Microvascular Network.- Stem Cell Bioprinting.