This book constitutes the refereed proceedings of the 8th Brazilian Symposium on Bioinformatics, BSB 2013, held in Recife, Brazil, in November 2013. The 18 regular papers presented were carefully reviewed and selected for inclusion in this book. The papers cover all aspects of bioinformatics and computational biology.

The Trouble with Long-Range Base Pairs in RNA Folding.- Roles of RORa on Transcriptional Expressions in the Mammalian Circadian Regulatory System.- HybHap: A Fast and Accurate Hybrid Approach for Haplotype Inference on Large Datasets.- Restricted DCJ-Indel Model Revisited.- MSA-GPU: Exact Multiple Sequence Alignment Using GPU.- MultiSETTER - Multiple RNA Structure Similarity Algorithm.- Assessing the Accuracy of the SIRAH Force Field to Model DNA at Coarse Grain Level.- How to Multiply Dynamic Programming Algorithms.- Influence of Scaffold Stability and Electrostatics on Top7-Based Engineered Helical HIV-1 Epitopes.- Random Forest and Gene Networks for Association of SNPs to Alzheimer's Disease.- Multilayer Cluster Heat Map Visualizing Biological Tensor Data.- On the 1.375-Approximation Algorithm for Sorting by Transpositions in O(n log n) Time.- ncRNA-Agents: A Multiagent System for Non-coding RNA Annotation.- Inference of Genetic Regulatory Networks Using an Estimation of Distribution Algorithm.- A Network-Based Meta-analysis Strategy for the Selection of Potential Gene Modules in Type 2 Diabetes.- RAIDER: Rapid Ab Initio Detection of Elementary Repeats.- A Probabilistic Model Checking Analysis of the Potassium Reactions with the Palytoxin and Na+/K+-ATPase Complex.- False Discovery Rate for Homology Searches.- A Pipeline to Characterize Virulence Factors in Mycobacterium Massiliense Genome.