The chapter "2D SDS PAGE in Combination with Western Blotting and Mass Spectrometry Is a Robust Method for Protein Analysis with Many Applications" is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Mass Spectrometry for Proteomics-based Investigation.- MALDI profiling and applications in medicine.- Applications of Matrix-Assisted Laser Desorption Ionization In-Source Decay Mass Spectrometry.- Proteome imaging: from classic to modern mass spectrometry-based molecular histology.- Mass Spectrometry-Based Tissue Imaging of Small Molecules.- Sequencing Proteins from Bottom to Top: Combining Techniques for Full Sequence Analysis of Glucokinase.- Proteomics and non-proteomics approaches to study stable and transient protein-protein interactions.- Contribution of Mass spectrometry-based Proteomics to Discoveries in Developmental Biology.- Profiling optimal conditions for capturing EDEM proteins complexes in melanoma using mass spectrometry.- The Methods employed in Mass Spectrometric Analysis of Posttranslational Modifications (PTMs) and Protein-Protein Interactions (PPIs).- Identification of post-translational modifications (PTMs) of proteins by mass spectrometry.- Sequence Variant and Post-translational Modification Analysis During Cell Line Selection via High-throughput Peptide Mapping.- Selective derivatization of hexahistidine-tagged recombinant proteins.- Investigation of Antibody-Drug Conjugates by Mass Spectrometry.- Mass Spectrometry- and Computational Structural Biology-Based Investigation of Proteins and Peptides.- Strategies for Membrane Protein Analysis by Mass Spectrometry.- The Potential for Ion Mobility in Pharmaceutical and Clinical Analyses.- Application of Ion Mobility Mass Spectrometry in Lipidomics.- Mass Spectrometry in Advancement of Redox Precision Medicine.- Applications for mass spectrometry in the study of ion channel structure and function.- Immuno - affinity Mass Spectrometry: A Novel Approaches with Biomedical Relevance.- Developing well-annotated species-specific protein databases using comparative proteogenomics.- Stoichiometry of heavy metal binding to peptides involved in Alzheimer's disease: mass spectrometric evidence.- Role of mass spectrometry in investigating a novel protein: the example of Tumor Differentiation Factor (TDF).- Mass Spectrometry-Based Biomarkers in Drug Developmen.- Combinatorial Electrophoresis and Mass Spectrometry-Based Proteomics in Breast Milk for Breast Cancer Biomarker Discovery.- Proteomic approaches to dissect neuronal signalling pathways.- Mass Spectrometry for the Study of Autism and Neurodevelopmental Disorders.- Simplifying the proteome: Analytical strategies for improving peak capacity.- Exploration of nicotine metabolism in Paenarthrobacter nicotinovorans pAO1 by microbial proteomics.- Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC) for Quantitative Proteomics.- Mass spectrometry based comparative proteomics using one dimensional and two dimensional SDS-PAGE of rat atria induced with obstructive sleep apnea.- 2D SDS PAGE in combination with western blotting and mass spectrometry is a robust method for protein analysis with many applications.- Pulse SILAC approaches to the measurement of cellular dynamics.- Protein Biomarkers in Major Depressive Disorder: an Update.- A Pilot Exploratory Proteomics Investigation of Mental Fatigue and Mental Energy.- Metabolomics and biomarker discovery.- Analytical Methods for Mass Spectrometry-Based Metabolomics Studies.- Trends in analysis of cortisol and its derivatives.- Detection of biomedically relevant stilbenes from wines by mass spectrometry.- Mass spectrometry as a complementary approach for noncovalently bound complexes based on cyclodextrins.- Trends in glycolipid biomarker discovery in neurodegenerative disorders by mass spectrometry.- Non-targeted Screening in Environmental Monitoring Programs.- Mass Spectrometric DNA Adduct Quantification by Multiple Reaction Monitoring and its Future Use for the Molecular Epidemiology of Cancer.- Bottlenecks in proteomics: an update.- Recent Applications of Mass Spectrometry at Clarkson University.- Index.