This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.

Activity-based protein pröling - enabling multimodal functional studies of microbial communities.- Activity-Based Protein Pröling Methods to Study Bacteria: The Power of Small-Molecule Electrophiles.- Opportunities and Challenges in Activity-Based Protein Pröling of Mycobacteria.- Activity-Based Protein Pröling at the Host-Pathogen Interface.- Chemical Proteomic Pröling of Protein Fatty-Acylation in Microbial Pathogens.- How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling.- ABPP and Host-Virus Interactions.- Activity-Based Protein Pröling for the Study of Parasite Biology.- Deciphering T Cell Immunometabolism with Activity-Based Protein Pröling.- Small-Molecule Inhibitors of PARPs: From Tools for Investigating ADP-Ribosylation to Therapeutics.- Development of Activity-Based Proteomic Probes for Protein Citrullination.- Recent Advances in Activity-Based Protein Pröling of Proteases.- Opportunities for Lipid-Based Probes in the Field of Immunology.- Activity-Based Protein Pröling of Non-ribosomal Peptide Synthetases.- Target Identi¿cation of Bioactive Covalently Acting Natural Products.- Applications of Reactive Cysteine Pröling.