Comprehensive, systematic review of advances in key CRISPR/Cas technologies, such as TALENS and zinc finger nucleases, double-strand break repair techniques, insertion-based genome edits, base editing, guide RNAs, gRNA/Cas9 constructs and CRISPR/Cas off targeting

Part 1 Genome editing techniques1.Using TALENs for genome editing in plants: Hilal Betul Kaya, Manisa Celal Bayar University, Turkey; Rhitu Rai, ICAR-National Institute for Plant Biotechnology, India; and Adam J. Bogdanove, Cornell University, USA; 2.Double strand break (DSB) repair pathways in plants and their application in genome engineering: Natalja Beying, Carla Schmidt and Holger Puchta, Karlsruhe Institute of Technology, Germany; 3.Advances in the generation of insertion-based genome edits in plants: Baike Wang and Juan Wang, Institute of Horticulture Crops - Xinjiang Academy of Agricultural Sciences, China; Shaoyong Huang, Institute of Horticulture Crops - Xinjiang Academy of Agricultural Sciences and College of Forestry and Horticulture - Xinjiang Agricultural University, China; and Yaping Tang, Ning Li, Shengbao Yang, Tao Yang and Qinghui Yu, Institute of Horticulture Crops - Xinjiang Academy of Agricultural Sciences, China; 4.Viruses as vectors for the delivery of gene-editing reagents: Evan E. Ellison, James C. Chamness and Daniel F. Voytas, University of Minnesota, USA; 5.Progress in precise and predictable genome editing in plants with base editing: Sabine Fräbel, Shai J. Lawit, Jingyi Nie, David G. Schwark, Thomas J. Poorten and Nathaniel D. Graham, Pairwise Plants, USA; 6.Advances in guide RNA design for editing plant genomes using CRISPR-Cas systems: Kaiyuan Chen, Hao Liu and Kabin Xie, Huazhong Agricultural University, China; Muhammad Tahir ul Qamar and Ling-Ling Chen, Huazhong Agricultural University, and Guangxi University, China; 7.Advances in assembling gRNA/Cas9 constructs in genome editing of plants: Marta Vázquez Vilar, Sara Selma, Asun Fernández del Carmen and Diego Orzáez, Instituto de Biología Molecular and Celular de Plantas de Valencia (CSIC-UPV), Spain; 8.Strategies for CRISPR/Cas9-mediated genome editing: from delivery to production of modified plants: William Gordon-Kamm, Pierluigi Barone, Sergei Svitashev, Jeffry D. Sander, Sandeep Kumar and Todd Jones, Corteva Agriscience, USA; 9.Advances in screening plants for edits and off-targets: Chun Wang and Kejian Wang, China National Rice Research Institute, China; 10.Targeted modification of promoters: Andika Gunadi and Ning Zhang, Boyce Thompson Institute, USA; and John J. Finer, The Ohio State University, USA; 11.The regulation of genome-edited crops: Gregory Jaffe, Center for Science in the Public Interest, USA; Part 2 Applications12.Genome editing of barley: Martin Becker, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany; and Goetz Hensel, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), Germany, and Palacký University, Czech Republic; 13.Genome editing of maize: Jacob D. Zobrist, Morgan McCaw, Minjeong Kang, Alan L. Eggenberger, Keunsub Lee, and Kan Wang, Iowa State University, USA; 14.Genome editing of sorghum: Aixia Li, Shandong University, China; and David R. Holding, University of Nebraska-Lincoln, USA; 15.CRISPR/Cas9-mediated genome editing in Brassica: Cheng Dai, Xia Tian and Chaozhi Ma, Huazhong Agricultural University, China; 16.Genome editing of tomatoes and other Solanaceae: Joyce Van Eck, The Boyce Thompson Institute and Cornell University, USA; 17.Genome editing of woody perennial trees: Chung-Jui Tsai, University of Georgia, USA;