The second edition of Biological Aging:  Methods and Protocols expands upon the previous edition with current, detailed, useful and promising methods currently available to study aging. With new chapters on protocols that detail aging cell culture as well as many more contemporary approaches such as nuclear transfer, microarray and proteomics technologies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Cell Senescence Culturing Methods.- Digital Image Analysis of Cells Stained with the Senescence-Associated ¿-Galactosidase Assay.- Analysis of Biomarkers of Caloric Restriction in Aging Cells.- Cell Sorting of Young and Senescent Cells.- Studying the Replicative Life Span of Yeast Cells.- Methods for Creating Mutations in C. elegans that Extend Lifespan.- Aging Studies in Drosophila Melanogaster.- The Use of Calorie Restriction Mimetics to Study Aging.- Using Somatic-Cell Nuclear Transfer to Study Aging.- Induction of Cellular Senescence by Oncogenic RAS.- Methods of Cellular Senescence Induction Using Oxidative Stress.- Methods of Testing Pharmacological Drugs Effects on Aging and Life-span in Mice.- Mitochondria-targeted Anti-aging Gene Therapy with Adeno-associated Viral Vectors.- Real-Time Bioluminescence Functional Imaging for Monitoring Tissue Formation and Regeneration.- Exometabolomic Mapping of Caenorhabditis elegans - A Tool to Non-Invasively Investigate Aging.- Detecting Polymorphisms in Human Longevity Studies: HLA typing and SNP Genotyping by Amplicon Sequencing.- Gel Electrophoresis-based Proteomics of Senescent Tissues.- Single-Cell Semiconductor Sequencing.- Application of DNA Microarray Technology to Gerontological Studies.- Epigenetic Biomarker to Determine Replicative Senescence of Cultured Cells.- Single-Neuron Transcriptome and Methylome Sequencing for Epigenomic Analysis of Aging.