Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.Comprehensive and innovative, Plant Genomics: Methods and Protocols is an essential guide for all plant scientists who are interested in further studies in the area of genomics research.

Provides insight into use of model plant species for genomics

Plant Genomics: Methods and ProtocolsDaryl J. Somers, Peter Langridge, and J. P. Gustafson, eds.Table of ContentsPrefaceContributors1. Role of Model Plant SpeciesRichard Flavell2. New Technologies for Ultra-High Throughput Genotyping in PlantsNikki Appleby; David Edwards; Jacqueline Batley3. Genetic Maps and the Use of SyntenyChris Duran; David Edwards; Jacqueline Batley4. A Simple TAE-based Method to Generate Large Insert BAC Libraries from Plant SpeciesBu-Jun Shi; J. Perry Gustafson; Peter Langridge5. Transcript Profiling and Expression Level MappingElena Potokina; Arnis Druka; Michael J. Kearsey6. Methods for Functional Proteomic AnalysesChristof Rampitsch; Natalia V. Bykova7. Stable Transformation of PlantsHuw D. Jones; Caroline A. Sparks8. Transient Transformation of PlantsHuw D. Jones; Angela Doherty; Caroline A. Sparks9. Bridging the Gene-to-Function Knowledge Gap Through Functional GenomicsStephen J. Robinson; Isobel A. P. Parkin10. Heterologous and Cell Free Protein Expression SystemsNaser Farrokhi; Maria Hrmova; Rachel A. Burton; Geoffrey B. Fincher11. Functional Genomics and Structural Biology in the Definition of Gene FunctionMaria Hrmova; Geoffrey B. Fincher12. In situ Analysis of Gene Expression in PlantsSinéad Drea; Paul Derbyshire; Rachil Koumproglou; Liam Dolan; John H. Doonan; Peter Shaw13. Plant and Crop DatabasesDavid E. Matthews; Gerard R. Lazo; Olin D. Anderson14. Plant Genome Annotation MethodsShu Ouyang; Françoise Thibaud-Nissen; Kevin L. Childs; Wei Zhu; C. Robin Buell15. Molecular Plant Breeding:Methodology and AchievementsRajeev K. Varshney; Dave A. Hoisington; Spurthi N. Nayak; Andreas Graner16. Practical Delivery of Genes to the MarketplaceDavid A. Fischhoff; Molly N. Cline17. Ecological Genomics of Natural Plant Populations: The Israeli PerspectiveEviatar Nevo18. Genome Sequencing Approaches and SuccessesMichael Imelfort; Jacqueline Batley; Sean Grimmond; David Edwards