Here is a compendium of data pertinent to the methods and protocols that have contributed to both recent advances in molecular medicine in general as well as to molecular basis of rheumatic disease in particular. This two-volume work collects the contributions of leaders in the field who cover such exciting and cutting edge topics as imaging and immunohistochemistry, analysis of cartilage and bone catabolism, immunobiology, and cell trafficking.

Broad range of topics covering many aspects of rheumatic diseaseIn-depth studies of cutting-edge technologyRobust tools for translational researchContributions from leaders in their fields

Arthritis: Methods and Protocols, Volume IIAndrew P. Cope, PhDI. Immunobiology1. Phenotypic Analysis of B Cells and Plasma CellsHenrik E. Mei, Taketoshi Yoshida, Gwendowlin Muehlinghaus, Falk Hiepe Thomas Dorner, Andreas Radbruch and Bimba F. Hoyer2. Detection of Antigen Specific B Cells in TissuesMarie Wahren-Herlenius and Stina Salomonsson3. Single Cell Analysis of Synovial Tissue B CellsHye-Jung Kim and Claudia Berek4. Tracking Antigen Specific CD4+ T Cells with Soluble MHC MoleculesJohn A. Gebe and William W. Kwok5. Analysis of Antigen Reactive T CellsClare Alexander, Richard C. Duggleby, Jane C. Goodall, Malgosia K. Matyszak, Natasha Telyatnikovaand J.S. Hill Gaston6. Identification and Manipulation of Antigen Specific T Cells with Artificial Antigen Presenting CellsEva Koffeman, Elissa Keogh, Mark Klein, Berent Prakken and Salvatore Albani7. Analysis of Th1/Th2 T Cell SubsetsAlla Skapenko and Hendrik Schulze-Koops8. Analysis of the T Cell Receptor Repertoire of Synovial T CellsLucy Wedderburn and Douglas J. King9. The Assessment of T Cell Apoptosis in Synovial FluidKarim Razza, Dagmar Scheel-Toellner, Janet M. Lord, Arne N. Akbar, Christopher D. Buckley and Mike Salmon10. Assay of T Cell Contact Dependent Monocyte-Macrophage FunctionsDanielle Burger and Jean-Michel Dayer11. Phenotypic and Functional Analysis of Synovial Natural Killer CellsNicola Dalbeth and Margaret F. C. Callan12. Identification and Isolation and Synovial Dendritic CellsAllison R Pettit, Lois Cavanagh, Amanda Boyce, Jagadish Padmanabha, Judy Peng and Ranjeny ThomasII. Animal Models of Arthritis13. The Use of Animal Models for Rheumatoid ArthritisRikard Holmdahl14. Collagen Induced Arthritis in MiceRichard O. Williams15. Collagen Induced Arthritis in RatsMarie M. Griffiths, Grant W. Cannon, Tim Corsi, Van Reese and Kandie Kunzler16. Collagen Antibody Induced Arthritis (CAIA)Kutty Selva Nandakumar and Rikard Holmdahl17. Arthritis Induced with Minor Cartilage ProteinsStefan Carlsen, Shemin Lu and Rikard Holmdahl18. Murine Antigen-Induced ArthritisWim van den Berg, Leo A. B. Joosten, and Peter L.E.M. van Lent19. Pristane Induced Arthritis in the RatPeter Olofsson and Rikard Holmdahl20. The K/BxN Mouse Model of Inflammatory Arthritis: Theory and PracticePaul Monach, Kimie Hattori, Haochu Huang, Elzbieta Hyatt, Jody Morse1, Linh Nguyen, Adriana Ortiz-Lopez, Hsin-Jung Wu, Diane Mathis, and Christophe Benoist21. Analysis of Arthritic Lesions in the Del1 Mouse: A Model for OAAnna-Marja Säämänen, Mika Hyttinen and Eero VuorioIII. Application of New Technologies to Define Novel Therapeutic Targets22. Gene Expression Profiling in RheumatologyTineke C.M.T. van der Pauw Kraan, Lisa G.M. van Baarsen, Francois Rustenburg, Belinda Baltus, Mike Fero and Cornelis L. Verweij23. Differential Display Reverse Transcription-Polymerase Chain Reaction (DDRT-PCR) to Identify Novel Biomolecules in Arthritis ResearchManir Ali and John D. Isaacs24. Two-Dimensional Electrophoresis of Proteins Secreted from Articular CartilageMonika Hermansson, Jeremy Saklatvala and Robin Wait25. Mapping Lymphocyte Plasma Membrane Proteins: A Proteomic ApproachMatthew J. Peirce, Jeremy Saklatvala, Robin Wait and Andrew P. Cope26. In Vivo Phage Display Selection in the Human/SCID Mouse Chimera Model for Defining Synovial Specific DeterminantsLewis Lee, Toby Garrood and Costantino Pitzalis27. Adenoviral Targeting of Signal Transduction Pathways in Synovial Cell