Microchip-Based Assay Systems explores recent progress in the microelectronics arena, the resultant miniaturization of component device features to nanometer size particles, and the ensuing growth in the development and use of microchip-based techniques in leading laboratories around the world. While the area of microelectronics faces daunting challenges, the field also promises fascinating new advances that may translate into products such as sensors and devices that use nanometer-sized building blocks for real-world applications. The contributors to this volume are part of growing interdisciplinary efforts among pioneers in microelectronics, nanoscience, and health that will continue to create exciting new microchip-based applications across a variety of sectors. In the 16 chapters of this volume, leading scientists present technically precise microchip-based assays for a variety of applications. The protocols detailed in this volume will help foster interest in microchip-based assays by providing readers with all the tools necessary to create their own microchip-based assays targeted to new applications. Certainly, Microchip-Based Assay Systems will help to expand the scope and accelerate the transition of microchip-based assays from academic and industrial research and development centers to real-world use.

Table of Contents / Microchip-based Assay Systems / Ed. Pierre N. FlorianoMicrochip Electrophoresis for DNA Separation by Wire-Imprinted Microchannels on PMMA SubstratesShu-Hui ChenFabrication of Porous Polymer Monoliths in Microfluidic Chips for Selective Nucleic Acid Concentration and PurificationJay A. A. West, Brent C. SatterfieldRapid Electrical Lysis of Bacterial Cells in a Microfluidic DeviceHsiang-Yu Wang, Padmapriya P. Banada, Dr. Arun Bhunia , Chang LuOn-Chip Bioassay Using Immobilized Sensing Bacteria in Three-Dimensional Microfluidic NetworkHirofumi Tani, Koji Maehana, Tamio KamidateMicrochip-based Enumeration of Human White Blood CellsPierre Floriano, Shelley Acosta, Nick Christodoulides, John T. McDevittMicrochip for the Diagnosis of Cervical CancerAnja Gulliksen, Frank KarlsenDNA Microchips Towards Molecular Signatures in Cervical CancersYick F. Wong, Tony K.H. Chung, Vivian W. Wang, David I. SmithImpedimetric-Detection for DNA Hybridization within Microfluidic BiochipsLouise Lingerfelt, James Karlinsey, James P. Landers, Anthony Guiseppi-ElieApplications of Functional Protein Microarrays: Identifying Protein-Protein Interactions in an Array FormatMatthew A. Coleman, Peter T. Beernink, Julio A. Camarero, Joanna S AlbalaA Microchip-based Assay for Interleukin-6Nicolaos Christodoulides, Prya Dharsham, Jorge Wong, Pierre N. Floriano , Dean Neikirk , John T. McDevittAllergen Microarrays for the Diagnosis of Specific IgE Against Components of Cow milk and Hen Egg in a Multiplex Biochip-based ImmunoassayChristian Harwanegg, Sabine Hutter, Reinhard HillerSurface Plasmon Resonance Imaging on Polypyrrole Protein Chips: Application to Streptavidin Immobilization and ImmunodetectionEmilie MERCEY, Ludivine GROSJEAN, Andre Roget, ThierryLIVACHEProtein Array-based Multiplexed Cytokine AssaysCheng C. WangLectin Microarrays for Glycoprotein AnalysisLara K. Mahal, Kanoelani PilobelloInteraction of HIV RNA with Peptides Detected by Acoustic Shear Wave Sensor Operated in an On-line FormatAnil Deisingh, Satie Siewah , Nardos Tassew, Michael ThompsonMicrochip-based Electrochemical Enzyme ImmunoassaysMadhu Prakash Chatrathi, Greg Collins, Joseph Wang