This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.

Cutting edge methods that employ monoclonal antibodies in a clinical settingIn-depth description for generating mouse and recombinant humanized antibodiesComprehensive review of how antibodies are being used in bead-based methods for measuring proteinsReviews of both flow cytometry and immunohistochemistry methods in diagnosis and classification of hematologic diseases

Table of Contents1. Development and Characterization of Mouse Hybridomas.2. Recombinant Monoclonal Antibodies.3. Soluble Fc Fusion Proteins for Biomedical Research.4. The use of the Antibodies in the Diagnosis of Leukemia and Lymphoma by Flow Cytometry.5. Quantification of Surface Antigens and Quantitative Flow Cytometry.6. CD13 and CD10 expression of granulocytes as markers for the functioning of the immune system. Quantification of the expression of membrane molecules using 1:1 labeled monoclonal antibodies and flow cytometry.7. Monitoring Cell Signaling pathways by Quantitative Flow Cytometry.8. Antibodies and Immunohistochemical Evaluation for the Diagnosis of Hematologic Malignancies: An Overview9. Bead Based Multi-Analyte Flow Immunoassays: The Cytometric Bead Array (CBA) System.10. Cell-Free bead-based Detection of Total and Phosphorylated Proteins in Plasma and Cell Lysate.11. Measuring Humanized Antibodies in Plasma of Patients Treated with Antibody-based Therapy using Bead-based Flow Cytometry.12. Detection of Chromosome Translocations by Bead-based Flow Cytometry.13. Detection of the MDR1 P-Glycoprotein expression and function.14. Detection of human antibodies generated against therapeutic antibodies used in tumor therapy.15. Measuring Allergen-Specific IgE: Where have we been and where are we going?