A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Includes supplementary material: sn.pub/extras

Part I. IntroductionAn Introduction to Plant Cell Culture: Back to the FutureVictor M. Loyola-Vargas and Felipe Vázquez-FlotaHistory of Plant Tissue CultureTrevor A. ThorpePart II. Cell Culture and Plant Regeneration: The FundamentalsPathogen and Biological Contamination Management: The Road AheadAlan C. Cassells and Barbara M. DoyleGrowth Measurements: Estimation of Cell Division and Cell ExpansionGregorio Godoy-Hernández and Felipe A. Vázquez-FlotaCallus and Suspension Culture Induction, Maintenance, and CharacterizationAlejandrina Robledo-Paz, María Nélida, Vázquez-Sánchez, Rosa María Adame-Alvarez, and Alba Estela Jofre-GarfiasMeasurement of Cell Viability in In Vitro CulturesLizbeth A. Castro-Concha, Rosa María Escobedo, and María de Lourdes Miranda-HamCryopreservation of Embryogenic Cell Suspensions by Encapsulation-VitrificationQiaochun Wang and Avihai PerlSomatic Embryogenesis in Picea Suspension CulturesClaudio StasollaIndirect Somatic Embryogenesis in Cassava for Genetic Modification PurposesKrit Raemakers, Isolde Pereira, Herma Koehorst van Putten, and Richard VisserDirect Somatic Embryogenesis in Coffea canephoraFrancisco Quiroz-Figueroa, Miriam Monforte-González, Rosa M. Galaz-Ávalos, and Victor M. Loyola-VargasPart III. Plant Propagation In VitroA New Temporary Immersion Bioreactor System for MicropropagationManuel L. Robert, José Luis Herrera-Herrera, Gastón Herrera-Herrera, Miguel Ángel Herrera-Alamillo, and Pedro Fuentes-CarrilloProtocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex VitroGabriela Fuentes, Carlos Talavera, Yves Desjardins, and Jorge M. SantamaríaUse of Statistics in Plant BiotechnologyMichael E. ComptonAnEfficient Method for the Micropropagation of Agave SpeciesManuel L. Robert, José Luis Herrera-Herrera, Eduardo Castillo, Gabriel Ojeda, and Miguel Angel Herrera-AlamilloMicropropagation of Endangered Plant SpeciesZhihua Liao, Min Chen, Xiaofen Sun, and Kexuan TangClonal Propagation of SoftwoodsTrevor A. Thorpe, Indra S. Harry, and Edward C. YeungPart IV. Applications for Plant ProtoplastsIsolation, Culture, and Plant Regeneration From Leaf Protoplasts of PassifloraMichael R. Davey, Paul Anthony, J. Brian Power, and Kenneth C. LoweIsolation, Culture, and Plant Regeneration From Echinacea purpurea ProtoplastsZeng-guang Pan, Chun-zhao Liu, Susan J. Murch, and Praveen K. SaxenaProduction of Cybrids in Brassicaceae SpeciesMaksym Vasylenko, Olga Ovcharenko, Yuri Gleba, and Nikolay KuchukGuard Cell Protoplasts: Isolation, Culture, and Regeneration of PlantsGary TallmanProduction of Interspecific Hybrid Plants in PrimulaJuntaro Kato and Masahiro MiiPart V. Protocols for Genomic ManipulationAgrobacterium-Mediated Transformation of Petunia Leaf DiscsIngrid M. van der MeerTransformation of Wheat Via Particle BombardmentIndra K. Vasil and Vilma VasilChloroplast TransformationXiao-Mei Lu, Wei-Bo Yin, and Zan-Min HuThe Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection MarkersCésar De los Santos-Briones and Teresa Hernández-SotomayorTransformation of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector SystemZuo-yu Zhao and Jerry RanchPart VI. Accumulation of Metabolites in Plant CellsCapsaicin Accumulation in Capsicum spp. Suspension CulturesNeftalí Ochoa-AlejoIsolation and Purification of Ribosome-Inactivating ProteinsSang-Wook Park