An indispensable collection of updated classical and emerging techniques that promise to add critical knowledge to our understanding of cartilage metabolism in health and disease. Volume 1: Cellular and Molecular Tools describes proven molecular and cellular techniques for the in vitro study of normal and osteoarthritic cartilage through biochemical, biomolecular, immunological, and physical approaches, with emphasis on the genetic manipulation of cells. Volume 2: Structure and In Vivo Analysis, offers cutting-edge procedures for the study-at the tissue level-of turnover, structure, and functioning of normal and diseased articular cartilage by invasive and noninvasive means. Comprehensive and up-to-date, the two volumes of Cartilage and Osteoarthritis provide researchers and bench scientists with readily reproducible protocols for new experiments to understand-from the cellular to the animal level-the pathophysiology of cartilage and to discover molecular targets for pharmacological intervention.

Includes supplementary material: sn.pub/extras

Generation and Use of Transgenic Mice As Models of OsteoarthritisAnna-Marja Säämänen and Eero VuorioDevelopment and Clinical Application in Arthritis of a New Immunoassay for Serum Type IIA Procollagen NH2 PropeptideJean-Charles Rousseau, Linda J. Sandell, Pierre D. Delmas, and Patrick GarneroHistological and Immunohistological Studies on CartilageJames Melrose, Susan Smith, and Peter GhoshHistochemical Visualization of the Cartilage Hyaladherins Using a Biotinylated Hyaluronan Oligosaccharide Bioaffinity ProbeJames Melrose and Susan SmithMethods for Cartilage and Subchondral Bone HistomorphometryPhilippe Pastoureau and Agnès ChomelLaser-Mediated Microdissection As a Tool for Molecular Analysis in ArthritisMartin Judex, Elena Neumann, Steffen Gay, and Ulf Müller-LadnerAnalysis of Protein Distribution in Cartilage Using Immunofluorescence and Laser Confocal Scanning MicroscopyStephan Soeder, Alexander Kuhlmann, and Thomas AignerMolecular and Biochemical Assays of Cartilage ComponentsCaroline D. HoemannMechanical Characterization of Native and Tissue-Engineered CartilageAlbert C. Chen, Stephen M. Klisch, Won C. Bae, Michele M. Temple, Kevin B. McGowan, Kenneth R. Gratz, Barbara L. Schumacher, and Robert L. SahNoninvasive Study of Human Cartilage Structure by MRIFelix EcksteinHigh-Resolution MRI Techniques for Studies in Small-Animal ModelsOlivier BeufHigh-Resolution Imaging of Osteoarthritis Using Microcomputed TomographyLydia Wachsmuth and Klaus EngelkeHigh-Resolution Ultrasonography for Analysis of Age- and Disease-Related Cartilage ChangesAmena Saïed and Pascal LaugierEvaluation of Cartilage Composition and Degradation by High-Resolution Magic-Angle Spinning Nuclear Magnetic ResonanceJürgen Schiller, Daniel Huster, BeateFuchs, Lama Naji, Jörn Kaufmann, and Klaus ArnoldPulsed-Field Gradient-Nuclear Magnetic Resonance (PFG NMR) to Measure the Diffusion of Ions and Polymers in Cartilage: Applications in Joint DiseasesJürgen Schiller, Lama Naji, Robert Trampel, Wilfred Ngwa, Robert Knauss, and Klaus ArnoldDynamics of Collagen in Articular Cartilage Studied by Solid-State NMR MethodsDaniel Huster, Jürgen Schiller, and Klaus ArnoldComputerized Protocol for Anatomical and Functional Studies of JointsSandra Martelli and Stefano ZaffagniniIndex