Expert laboratorians detail emerging methodologies for studying the cell cycle, its kinases, and kinase inhibitors. The authors focus the issues of gene expression in vivo and in vitro, the analysis of cyclin-dependent kinase inhibitors, protein degradation mediated by the proteosome, the analysis of the transformed cell phenotype, and innovative techniques to detect apoptosis. Many of the protocols are new, such as electron microsocopy to detect apoptosis and proteosome-mediated degradation, whereas others study the interactions between protein-protein, protein-DNA, and protein-RNA.

Includes supplementary material: sn.pub/extras

Part I. Analysis of Cyclins and Cyclin-Dependent KinasesThe Biology of Cyclins and Cyclin-Dependent Protein Kinases: An IntroductionLucio MieleIn Situ Immunofluorescence Analysis: Immunofluorescence MicroscopyAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinIn Situ Immunofluorescence Analysis: Analyzing RNA Synthesis by 5-Bromouridine-5'-Triphosphate LabelingAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinImmunofluorescence Analysis Using Epitope-Tagged Proteins: In Vitro SystemAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinAnalysis of In Vivo Gene Expression Using Epitope-Tagged ProteinsAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinChromatin ImmunoprecipitationAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinProtein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Electrophoretic Mobility Shift AssayAmjadJaved, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinProtein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I DigestionAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinProtein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: Ligation-Mediated Polymerase Chain ReactionAmjad Javed, Sayyed K. Zaidi, Soraya E. Gutierrez, Christopher J. Lengner, Kimberly S. Harrington, Hayk Hovhannisyan, Brian C. Cho, Jitesh Pratap, Shirwin M. Pockwinse, Martin Montecino, André J. van Wijnen, Jane B. Lian, Janet L. Stein, and Gary S. SteinAssays for Cyclin-Dependent Kinase InhibitorsAdrian M. Senderowicz. Protein Degradation Via the Proteosome, Henry Hoff, Hong Zhang, and Christian SellPart II. Analysis of the Factors Involved in Cell Cycle DeregulationThe Transformed PhenotypeHenry Hoff, Barbara Belletti, Hong Zhang, and Christian SellA Morphologic Approach to Detect Apoptosis Based on Electron MicroscopyMartyn K. White and Caterina CintiDetection of Apoptotic Deoxyribonucleic Acid Break by In Situ Nick TranslationCarmela Trimarchi, Dario La Sala, Alessandra Zamparelli, and Caterina CintiPart III. Cellular Response to Deoxyribonucleic Acid DamageInduction of Deoxyribonucleic Acid Damage by Alkylating AgentsSalvatore Cortellino, David P. Turner, Domenico Albino, and Alfonso BellacosaInduction of Deoxyribonucleic Acid Damage by g IrradiationSalvatore Cortellino, David P. Turner and Alfonso