Allergy: Methods and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols. The protocols cover a range of disciplines including allergy, immunology, cell biology, and histology, and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, and T regulatory cells.

Allergy: Methods and Protocols aims to assist the researcher in gaining insight into the molecular mechanisms involved in allergy by featuring an array of protocols. The protocols cover a range of disciplines including allergy, immunology, cell biology, and histology, and include methods to investigate the cellular response to allergens, cytokine profile, MHC restriction, and T regulatory cells.

List of chapters1. Understanding the molecular mechanisms of allergy - Meinir Jones2. T cell - primary culture from peripheral blood - MonikaRaulf-Heimsoth3. Production of T cell lines - Helga Kahlert4. Production of human T cell clones - Adrienne Verhoef5. Mapping of human T-cell epitopes of allergens - Thomas Zeiler andTuomas Virtanen6. Determining MHC restriction of T cell response - Mark Larche7. Short term culture of CD8 cells and intracellular cytokine staining Beejal Vyas and Alistair Noble8. Isolation, flow cytometric analysis and suppression assay ofCD4+CD25+ T regulatory cells - Hayley Jeal9. Monocyte-derived dendritic cells as antigen presenting cells in Tcell proliferation and cytokine production - Sun-sang J Sung10. Ultrasensitive ELISA for measurement of human cytokine responses inprimary culture - William P. Stefura, J. Darren Campbell, RenéeDouville, Monique J. Stinson, F. Estelle Simons, Allan B. Becker, andKent T. HayGlass11. Quantification of human chemokine production in TLR-stimulated andantigen-specific recall responses - Monique Stinson, Renee DouvilleYuriy Lissitsyn, Melanie Blanchard, William Stefura, Estelle SimonsAllan Becker, Peter Nickerson, Kevin Coombs, Kent HayGlass12. Standardisation of allergen extracts - Jørgen Nedergaard Larsen andSten Dreborg13. Immuno-electrophoresis for the characterisation of allergenextracts - Gitte Nordskov Hansen and Jørgen Nedergaard Larsen14. Conjugation of haptens - Ranulfo Lemus and Meryl H. Karol15. Monoclonal antibodies - Helga Kahlert and Oliver Cromwell16. Purification of antibodies - Per H Larsson and Anne Renström17. Collection of air samples to quantify exposure to airborneallergens - Susan Gordon18. Assay of air sample eluates - Anne Renström and Susan Gordon19. The halogen assay - a new technique for measuring airborne allergen Euan Tovey,Sandra De Lucca, Leanne Poulos and Tim O'Meara20. Measurement of specific IgG anti-Fel d 1 antibodies - Meinir Jones21. The facilitated-antigen binding (FAB) assay - a protocol to measureallergen-specific inhibitory activity - James N Francis22. Microscopic identification and purity determination of pollengrains - Magdalena Rahl23. Biopanning for the characterisation of allergen mimotopesIsabella Schöll and Erika Jensen-Jarolim24. Identification of mast cells and mast cell subpopulations - MarkBuckley and Andrew F Walls25. Purification and characterisation of mast cell tryptase and chymase Alan R McEuen and Andrew F Walls26. Experimental activation of mast cells and their pharmacologicalmodulation - Shaoheng He and Andrew F Walls27. In situ hybridisation - Kayhan T. Nouri-Aria