Recent outbreaks of swine influenza and avian influenza, along with the remaining and in some cases expanding threats from HIV, dengue virus, and the viruses causing hepatitis, have reinforced the need for rapid, accurate and cost-effective diagnosis of viral disease. Diagnostic Virology Protocols, Second Edition brings the field fully up-to-date with a focus on protocols involving nucleic acid detection, most often through some form of the polymerase chain reaction (PCR). The expert contributors also delve into the key technology of robotics as well as future prospects, such as further refined point-of-care testing and the increasing importance of mathematical modelling. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Diagnostic Virology Protocols, Second Edition captures the dramatic changes in the virus diagnostic laboratory in order to better prepare scientists to combat the inevitable threats to public health from future and present infectious diseases.

Highlights the major changes in the virus diagnostic laboratory

The Detection of Adenoviruses.- Alphaviruses.- Detection of Human Caliciviruses in Fecal Samples by RT-PCR.- The Detection of Dengue Virus.- Detection of Enteroviruses from Clinical Specimens.- The Detection of Hepatitis Viruses.- Molecular Detection of Herpesviruses.- HIV Drug Resistance Testing.- Detection, Quantification, and Characterization of HIV/SIV.- Simultaneous Molecular Detection and Confirmation of Influenza AH5, with Internal Control.- Detection of Measles, Mumps, and Rubella Viruses.- Detection of High-Risk Mucosal Human Papillomavirus DNA in Human Specimens by a Novel and Sensitive Multiplex PCR Method Combined with DNA Microarray.- The Detection of Parvoviruses.- The Detection and Characterization of Polioviruses.- Detection of Human-Pathogenic Poxviruses.- Lyssaviruses: Special Emphasis on Rabies Virus and Other Members of the Lyssavirus Genus.- Simultaneous Detection and Differentiation of Respiratory Syncytial Virus and Other Respiratory Viral Pathogens.- Rotaviruses.- Detection of Crimean-Congo Hemorrhagic Fever, Hanta, and Sandfly Fever Viruses by Real-Time RT-PCR.- Detection of SARS Coronavirus.- The Detection of West Nile Virus.- Point of Care Testing: Diagnosis Outside the Virology Laboratory.- Modelling Emerging Viral Epidemics for Public Health Protection.