Plant genomics is a growing and constantly evolving field of study, one which has gained much ground in past years through the development of advanced research and data management tools. In Plant Genomics: Methods and Protocols, expert researchers explore the current issues and methodologies of this expanding field, specifically addressing areas of gene discovery and the functional analysis of genes with a focus on the primary tools and sub-disciplines of genetic mapping, mRNA, protein and metabolite profiling. Chapters employ exciting new methods to investigate molecular plant breeding technology and gene functional analysis via transformation, mutation, protein function, and gene expression. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

Role of Model Plant Species.- New Technologies for Ultra-High Throughput Genotyping in Plants.- Genetic Maps and the Use of Synteny.- A Simple TAE-Based Method to Generate Large Insert BAC Libraries from Plant Species.- Transcript Profiling and Expression Level Mapping.- Methods for Functional Proteomic Analyses.- Stable Transformation of Plants.- Transient Transformation of Plants.- Bridging the Gene-to-Function Knowledge Gap Through Functional Genomics.- Heterologous and Cell-Free Protein Expression Systems.- Functional Genomics and Structural Biology in the Definition of Gene Function.- In situ Analysis of Gene Expression in Plants.- Plant and Crop Databases.- Plant Genome Annotation Methods.- Molecular Plant Breeding: Methodology and Achievements.- Practical Delivery of Genes to the Marketplace.- Ecological Genomics of Natural Plant Populations: The Israeli Perspective.- Genome Sequencing Approaches and Successes.