The second edition of Adhesion Protein Protocols combines traditional techniques with cutting-edge and novel techniques that can be adapted easily to different molecules and cell types. The topics discussed include novel techniques for studying cell-cell adhesion, neutrophil chemotaxis, in vitro assays used to study leukocyte migration through monolayers of cultured endothelial cells, and novel techniques to purify pseudopodia from migratory cells. The protocols discussed in this volume are suitable for both novice and expert scientists, who will gain further insight into the complex and incompletely understood processes involved in cellular adhesion.

Manipulation of Cell-Cell Adhesion Using Bowtie-Shaped Microwells.- Separation of Cell-Cell Adhesion Complexes by Differential Centrifugation.- Analysis of Neutrophil Chemotaxis.- Analysis of Leukocyte Migration Through Monolayers of Cultured Endothelial Cells.- Biochemical Purification of Pseudopodia from Migratory Cells.- Dynamic Assessment of Cell-Matrix Mechanical Interactions in Three-Dimensional Culture.- Quantitative Analyses of Cell Adhesion Strength.- Using RNA Interference to Knock Down the Adhesion Protein TES.- Assaying Calpain Activity.- Analysis of Focal Adhesions and Cytoskeleton by Custom Microarray.- Proteomic Analysis of Cell Surface Membrane Proteins in Leukemic Cells.- Bioinformatic Analysis of Adhesion Proteins.- Analysis of Integrin Dynamics by Fluorescence Recovery After Photobleaching.- Double-Hydrogel Substrate as a Model System for Three-Dimensional Cell Culture.- In Vitro Actin Assembly Assays and Purification From Acanthamoeba.