This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.

Development and Characterization of Mouse Hybridomas.- Recombinant Monoclonal Antibodies.- Soluble Fc Fusion Proteins for Biomedical Research.- The Use of the Antibodies in the Diagnosis of Leukemia and Lymphoma by Flow Cytometry.- Quantification of Surface Antigens and Quantitative Flow Cytometry.- CD13 and CD10 Expression of Granulocytes as Markers for the Functioning of the Immune System.- Monitoring Cell Signaling Pathways by Quantitative Flow Cytometry.- Antibodies and Immunohistochemical Evaluation for the Diagnosis of Hematological Malignancies.- Bead-Based Multianalyte Flow Immunoassays.- Cell-Free Bead-Based Detection of Total and Phosphorylated Proteins in Plasma and Cell Lysates.- Measuring Humanized Antibodies in Plasma of Patients Treated With Antibody-Based Therapy Using Bead-Based Flow Cytometry.- Detection of Chromosome Translocations by Bead-Based Flow Cytometry.- Detection of the MDR1 P-Glycoprotein Expression and Function.- Detection of Human Antibodies Generated Against Therapeutic Antibodies Used in Tumor Therapy.- Measuring Allergen-Specific IgE.