This second part in the two-volume work Microarrays details applications and data analysis. It includes insight into non-mammalian vertebrate systems, processes and protocols for high quality glass-based microarrays. Coverage includes applications in DNA, peptide, antibody and carbohydrate microarraying, oligonucleotide microarrays generated from hydrolysis PCR probe sequences, microarray platforms in clinical practice, and screening of cDNA libraries on glass slide microarrays. Authors in this volume also discuss protocols for predicting DNA duplex stability on oligonucleotide arrays and integrated analysis of microarray results.

The Use of Microarray Technology in Nonmammalian Vertebrate Systems.- Quality Considerations and Selection of Surface Chemistry for Glass-Based DNA, Peptide, Antibody, Carbohydrate, and Small Molecule Microarrays.- Optimization Workflow for the Processing of High Quality Glass-Based Microarrays.- Processing Protocols for High Quality Glass-Based Microarrays.- Specific Detection of Bacterial Pathogens Using Oligonucleotide Microarrays Generated From Hydrolysis PCR Probe Sequences.- Uses of Microarray Platforms in Cancer.- Microarray Technology for Use in Molecular Epidemiology.- Utilization of Microarray Platforms in Clinical Practice.- A Microarray-Based Method to Profile Global microRNA Expression in Human and Mouse.- Genotyping of Single Nucleotide Polymorphisms by Arrayed Primer Extension.- Protein Chip for Detection of DNA Mutations.- Screening of cDNA Libraries on Glass Slide Microarrays.- ArrayPlex SA.- Tumor-Stroma Interactions of Metastatic Prostate Cancer Cell Lines.- Identification of Small Molecule Targets on Functional Protein Microarrays.- Quantification of Small Molecules Using Microarray Technology.- Antibody-Microarrays on Hybrid Polymeric Thin Film-Coated Slides for Multiple-Protein Immunoassays.- Overprint Immunoassay Using Protein A Microarrays.- ?Paraflo(TM) Biochip for Nucleic Acid and Protein Analysis.- Application of ProteinChip Array Profiling in Serum Biomarker Discovery for Patients Suffering From Severe Acute Respiratory Syndrome.- Volumetric Mass Spectrometry Protein Arrays.- Microarray Data Classified by Artificial Neural Networks.- Methods for Microarray Data Analysis.- Predicting DNA Duplex Stability on Oligonucleotide Arrays.- Bioinformatics.- In Silico Gene Selection for Custom Oligonucleotide Microarray Design.- Integrated Analysis ofMicroarray Results.