Jeffrey Morgan and Martin Yarmush bring together in Tissue Engineering the first major collection of cutting-edge methods developed and used by leading researchers in the tissue engineering field. These hands-on experts describe easily reproducible protocols for the production and evaluation of polymers, scaffolds, and composites, as well as methods for the isolation, culture, and analysis of cells, including the combination of cells with a variety of materials or devices. Quantitative methods designed to assess cell performance and cell function that will also illuminate our understanding of cellular processes and the cellular response to the chemical and mechanical environment of a functional organ/tissue are also offered.Tissue Engineering provides researchers with detailed methods and protocols covering a comprehensive range of key technologies and techniques used by leaders in tissue engineering research. Its interdisciplinary methods-drawn from the life sciences, engineering, and clinical medicine-are already making a significant contribution to the development of new and effective treatments for both organ and tissue defects.

Materials.- Preparation of Collagen-Glycosaminoglycan Copolymers for Tissue Regeneration.- Preparation and Use of Tethered Ligands as Biomaterials and Tools for Cell Biology.- Nondestructive Evaluation of Biodegradable Porous Matrices for Tissue Engineering.- Fabrication of Biodegradable Polymer Foams for Cell Transplantation and Tissue Engineering.- Formation of Highly Porous Polymeric Foams with Controlled Release Capability.- Magnetic-Induced Alignment of Collagen Fibrils in Tissue Equivalents.- Preparation and Use of Thermosensitive Polymers.- In Situ Photopolymerized Hydrogels for Vascular and Peritoneal Wound Healing.- Fabrication and Implantation of Gel-Filled Nerve Guidance Channels.- Small Animal Surgical and Histological Procedures for Characterizing the Performance of Tissue-Engineered Bone Grafts.- Preparation and Use of Porous Poly(?-Hydroxyester) Scaffolds for Bone Tissue Engineering.- Cells.- Quantitative Assessment of Autocrine Cell Loops.- Measurement of Recovery of Function Following Whole Muscle Transfer, Myoblast Transfer, and Gene Therapy.- Preparation of Immortalized Human Chondrocyte Cell Lines.- Clinical Applications.- Isolation and In Vitro Proliferation of Chondrocytes, Tenocytes, and Ligament Cells.- Culture and Identification of Autologous Human Articular Chondrocytes for Implantation.- Organogenesis of Skeletal Muscle in Tissue Culture.- Hepatocyte Primary Cultures.- Formation and Characterization of Hepatocyte Spheroids.- Isolation and Culture of Human Endothelial Cells.- Isolation and Culture of Microvascular Endothelial Cells.- Initiation, Maintenance, and Quantification of Human Hematopoietic Cell Cultures.- Methods to Isolate, Culture, and Study Osteoblasts.- Cryopreservation of Rat Hepatocytes in a Three-Dimensional CultureConfiguration Using a Controlled-Rate Freezing Device.- Cell Material Composites.- Microencapsulation of Enzymes, Cells, and Genetically Engineered Microorganisms.- Methods for Microencapsulation with HEMA-MMA.- Micropatterning Cells in Tissue Engineering.- Methods for the Serum-Free Culture of Keratinocytes and Transplantation of Collagen-GAG-Based Skin Substitutes.- Use of Skin Equivalent Technology in a Wound Healing Model.- Preparation and Transplantation of a Composite Graft of Epidermal Keratinocytes on Acellular Dermis.- Development of a Bioartificial Liver Device.- Methods for the Implantation of Liver Cells.- Isolation and Long-Term Maintenance of Adult Rat Hepatocytes in Culture.- Design and Fabrication of a Small Caliber Hybrid Arterial Bioprosthesis.- Methods for the Immunoisolation and Transplantation of Pancreatic Cells.- Methods for the Study of Nerve Cell Migration and Patterning.- Measurement Techniques.- Estimating Number and Volume of Islets Transplanted Within a Planar Immunobarrier Diffusion Chamber.- Quantitative Measurement of Cell-Cell Adhesion Under Flow Conditions.- Quantitative Measurement of the Biological Response of Cartilage to Mechanical Deformation.- Measuring Receptor-Mediated Cell Adhesion Under Flow.- In Vitro and In Vivo Quantification of Adhesion Between Leukocytes and Vascular Endothelium.- Quantitative Measurement of Shear-Stress Effects on Endothelial Cells.- Quantitative Modeling of Limitations Caused by Diffusion.