Glycomics: Methods and Protocols
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Glycomics: Methods and Protocols

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Nicolle H. Packer
916 g
254x178x24 mm
2074, Methods in Molecular Biology

To aid scientists in a vital subject, this compendium of laboratory protocols reflects the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools designed for the analysis of glycosylation.
Presents a detailed compendium of techniques used routinely in the leading laboratories utilizing current, common technologies, especially those established for proteomics research
Section I: Glycan Analysis
Subsection A: Released Glycans

1. Analysis of N- and O-Linked Glycans from Glycoproteins Using Maldi-TOF Mass Spectrometry
Willy Morelle, Valegh Faid, Frédéric Chirat, and Jean-Claude Michalski

2. Infrared Multiphoton Dissociation (IRMPD) Mass Spectrometry for Structural Elucidation of Oligosaccharides
Bensheng Li, Hyun Joo An, Jerry L. Hedrick, and Carlito B. Lebrilla

3. Mass Spectrometry of N-linked Glycans
Parastoo Azadi and Christian Heiss

4. Solid-phase Permethylation for Glycomic Analysis
Yehia Mechref, Pilsoo Kang, and Milos V. Novotny

5. Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization
Erika Lattová and Hélène Perreault

6. 2-Dimensional HPLC Separation with Reverse Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools after Labeling with 2-Aminobenzamide
Manfred Wuhrer, Carolien A. M. Koeleman, and André M. Deelder

7. Capillary Lectin-Affinity Electrophoresis for Glycan Analysis
Kazuaki Kakehi and Mitsuhiro Kinoshita

8. Analysis of Methylated O-Glycan Alditols by CAD-ESI Mass Spectrometry
Franz-Georg Hanisch and Stefan Müller

9. High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling
Hasse Karlsson, Jessica M. Holmén Larsson, Kristina A. Thomsson, Iris Härd, Malin Bäckström, and Gunnar C. Hansson

10. Collision Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans
Bensheng Li, Hyun Joo An, Jerry L. Hedrick, and Carlito B. Lebrilla

11. The Structural Elucidation of Glycosaminoglycans
Vikas Prabhakar, Ishan Capila, and Ram Sasisekharan

12. Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescenceand Mass Tags for HPLC and MS Separations
Mark Skidmore, Abdel Atrih, Ed Yates, and Jeremy E. Turnbull

13. Small-Scale Enzymatic Digestion of Glycoproteins and Proteoglycans for Analysis of Oligosaccharides by LC-MS and FACE Gel Electrophoresis
Ruby P. Estrella, John M. Whitelock, Rebecca H. Roubin, Nicolle H. Packer, and Niclas G. Karlsson

Subsection B: Glycopeptides

14. Enrichment Strategies for Glycopeptides
Shigeyasu Ito, Ko Hayama, and Jun Hirabayashi

15. Introductory Glycosylation Analysis Using SDS-PAGE and Peptide Mass Fingerprinting
Nicole Wilson, Raina Simpson, and Catherine Cooper-Liddell

16. Characterization of N-Linked Glycosylation on Recombinant Glycoproteins Produced in Pichia pastoris Using ESI-MS and MALDI-TOF
Bing Gong, Michael Cukan, Richard Fisher, Huijuan Li, Terrance A. Stadheim, and Tillman Gerngross

17. N-Glycosylation Site Analysis of Human Platelet Proteins by Hydrazide Affinity Capturing and LC-MS/MS
Urs Lewandrowski and Albert Sickmann

18. LC/MSn For Glycoprotein Analysis: N-Linked Glycosylation Analysis And Peptide Sequencing Of Glycopeptides
Nana Kawasaki, Satsuki Itoh, and Teruhide Yamaguchi

Subsection C: O-GlcNAcomics

19. Detecting the 'O-GlcNAc-ome'; Detection, Purification, and Analysis of O-GlcNAc Modified Proteins
Natasha E. Zachara

Subsection D: Glycobioinformatic Tools

20. Preparation of a Glycan Library Using a Variety of Glycosyltrasferases
Hiromi Ito, Akihiko Kameyama, Takashi Sato, and Hisashi Narimatsu

21. Data Mining the PDB for Glyco-Related Data
Thomas Lütteke and Claus-W. von der Lieth

Section II: Carbohydrate-Protein
Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

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