The Proteomics Protocols Handbook
-9 %

The Proteomics Protocols Handbook

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ISBN-13:
9781588295934
Einband:
Book with CD-ROM
Seiten:
988
Autor:
John M. Walker
Gewicht:
3 g
Format:
254x178x mm
Sprache:
Englisch
Beschreibung:

Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification.
Extraction and Solubilization of Proteins for Proteomic Studies Richard M. Leimgruber

Preparation of Bacterial Samples for 2-D PAGE
Brian Berg Vandahl, Gunna Christiansen, and Svend Birkelund

Preparation of Yeast Samples for 2-D PAGE
Joakim Norbeck

Preparation of Mammalian Tissue Samples for Two-Dimensional Electrophoresis
Frank A. Witzmann

Differential Detergent Fractionation of Eukaryotic Cells
Melinda L. Ramsby and Gregory S. Makowski

Serum or Plasma Sample Preparation for Two-Dimensional Gel Electrophoresis
Anthony G. Sullivan, Stephen Russell, Henry Brzeski, Richard I. Somiari, and Craig D. Shriver

Preparation of Plant Protein Samples for 2-D PAGE
David W. M. Leung

Laser-Assisted Microdissection in Proteomic Analyses
Darrell L. Ellsworth, Stephen Russell, Brenda Deyarmin, Anthony G. Sullivan, Henry Brzeski, Richard I. Somiari, and Craig D. Shriver

Purification of Cellular and Organelle Populations by Fluorescence-Activated Cell Sorting for Proteome Analysis
William L. Godfrey, Colette J. Rudd, Sujata Iyer, and Diether Recktenwald

Purification of Nucleoli From Lymphoma Cells and Solubilization of Nucleolar Proteins for 2-DE Separation
Régis Dieckmann, Yohann Couté, Denis Hochstrasser, Jean-Jacques Diaz, and Jean-Charles Sanchez

Prefractionation of Complex Protein Mixture for 2-D PAGE Using Reversed-Phase Liquid Chromatography
Volker Badock and Albrecht Otto

Fractionation of Complex Proteomes by Microscale Solution Isoelectrofocusing Using ZOOM(TM) IEF Fractionators to Improve Protein Profiling
Xun Zuo, Ki-Boom Lee, and David W. Speicher

Large-Format 2-D Polyacrylamide Gel Electrophoresis
Henry Brzeski, Stephen Russell, Anthony G. Sullivan, Richard I. Somiari, and Craig D. Shiver

Analysis of Membrane Proteins by Two-Dimensional Gels
MichaelFountoulakis

2-D PAGE of High-Molecular-Mass Proteins
Masamichi Oh-Ishi and Tadakazu Maeda

Using Ultra-Zoom Gels for High-Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis
Sjouke Hoving, Hans Voshol, and Jan van Oostrum

NEpHGE and pI Strip Proteomic 2-D Gel Electrophoretic Mapping of Lipid-Rich Membranes
Steven E. Pfeiffer, Yoshihide Yamaguchi, Cecilia B. Marta, Rashmi Bansal, and Christopher M. Taylor

Silver Staining of 2-D Gels
Julia Poland, Thierry Rabilloud, and Pranav Sinha

Zn2+ Reverse Staining Technique
Carlos Fernandez-Patron

Multiplexed Proteomics Technology for the Fluorescence Detection of Glycoprotein Levels and Protein Expression Levels Using Pro-Q® Emerald and SYPRO® Ruby Dyes
Birte Schulenberg and Wayne F. Patton

Multiplexed Proteomics Technology for the Fluorescence Detection of Phosphorylation and Protein Expression Levels Using Pro-Q® Diamond and SYPRO® Ruby Dyes
Birte Schulenberg, Terrie Goodman, Thomas H. Steinberg, and Wayne F. Patton

Sensitive Quantitative Fluorescence Detection of Proteins in Gels Using SYPRO® Ruby Protein Gel Stain
Birte Schulenberg, Nancy Ahnert, and Wayne F. Patton

Rapid, Sensitive Detection of Proteins in Minigels With Fluorescent Dyes: Coomassie Fluor Orange, SYPRO® Orange, SYPRO Red, and SYPRO Tangerine Protein Gel Stains
Thomas H. Steinberg, Courtenay R. Hart, and Wayne F. Patton

Differential In-Gel Electrophoresis in a High-Throughput Environment
Richard I. Somiari, Stephen Russell, Stella B. Somiari, Anthony G. Sullivan, Darrell L. Ellsworth, Henry Brzeski, and Craig D. Shriver

Statistical Analysis of 2-D Gel Patterns
Françoise Seillier-Moiseiwitsch

2-DE Databases on the World Wide Web
Christine Hoogland, Khaled Mostaguir, and Ron D. Appel

Computer Analysis of 2-D Images
Patricia M. Palagi,
Hands-on researchers describe in step-by-step detail 73 proven laboratory methods and bioinformatics tools essential for analysis of the proteome. These cutting-edge techniques address such important tasks as sample preparation, 2D-PAGE, gel staining, mass spectrometry, and post-translational modification. There are also readily reproducible methods for protein expression profiling, identifying protein-protein interactions, and protein chip technology, as well as a range of newly developed methodologies for determining the structure and function of a protein. The bioinformatics tools include those for analyzing 2D-GEL patterns, protein modeling, and protein identification. All laboratory-based protocols follow the successful Methods in Molecular Biology(TM) series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

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