This volume reviews the increasing importance of glycosylation to the field of virology, as well as virus replication. The chapters provide an overview of glycosylation in relation to virus infection, and the generic techniques that are used to analyze and characterize glycoproteins. The information presented here provides insight as to how the techniques of glycobiology can be applied in virology and answer most questions that are of interest to the reader.

An introductory overview of viruses and glycosylation

Viruses and Glycosylation.- Interaction Between Respiratory Syncytial Virus and Glycosaminoglycans, Including Heparan Sulfate.- Expression of the Severe Acute Respiratory Syndrome Coronavirus 3a Protein and the Assembly of Coronavirus-Like Particles in the Baculovirus Expression System.- The C Type Lectins DC-SIGN and L-SIGN.- Functional Analysis of the N-Linked Glycans Within the Fusion Protein of Respiratory Syncytial Virus.- Expression and Purification of Viral Glycoproteins Using Recombinant Vaccinia Viruses for Functional and Structural Studies.- The Use of Two-Dimensional SDS-PAGE to Analyze the Glycan Heterogeneity of the Respiratory Syncytial Virus Fusion Protein.- The Use of Monoclonal Antibodies and Lectins to Identify Changes in Viral Glycoproteins That are Influenced by Glycosylation.- Expression, Glycosylation, and Modification of the Spike (S) Glycoprotein of SARS CoV.- Analysis of Glycoproteins of Viruses in the Family Bunyaviridae.- Secretion of the Respiratory Syncytial Virus Fusion Protein From Insect Cells Using the Baculovirus Expression System.- Characterization of the Dengue Virus Envelope Glycoprotein Expressed in Pichia pastoris.- Cloning, Expression, and Functional Analysis of Patient-Derived Hepatitis C Virus Glycoproteins.