T Cell Protocols

Besorgungstitel | Lieferzeit:3-5 Tage I
Gennaro De Libero
661 g
260x193x17 mm

Fully updated and ready to use, this second edition is an ideal guide for young investigators new to the complexities of immunology, and a valuable resource for experienced scientists wanting clear descriptions of new methods in this vital field of study.
Provides an easily accessible reference volume for cell-based phenotypic screening, written by leading researchers in the field
Chapter 1: Analysis of Frequency and Phenotype of Antigen-Specific T CellsAngus Stock and Vincenzo Cerundolo

Chapter 2: B Cell Helper Assays
Sergio Abrignani, Elena Tonti, Giulia Casorati, and Paolo Dellabona

Chapter 3: Trans-Kingdom RNA Interference (tkRNAi): A Novel Method to Induce Therapeutic Gene Silencing
Thu A. Nguyen and Johannes H. Fruehauf

Chapter 4: Flow Cytometry and Cell Activation
Sonia Gavasso

Chapter 5: Investigating T Cells by Polychromatic Flow Cytometry
Enrico Lugli, Leonarda Troiano, and Andrea Cossarizza

Chapter 6: Generation of Human T Cell Clones
Sabrina Mariotti and Roberto Nisini

Chapter 7: Limiting Dilution Analysis of Antigen-Specific T cells
Jorge Carneiro, Lurdes Duarte, and Elisabetta Padovan

Chapter 8: T Cell Epitope-Mapping by Cytokine Gene Expression Assay
Maurizio Provengano and Giulio C. Spagnoli

Chapter 9: Cytokine Multiplex Immunoassay: Methodology and (Clinical) Applications
Wilco de Jager, Berent Prakken, and Ger T. Rijkers

Chapter 10: Purification of the T Cell Antigen Receptor and Analysis by Blue-Native PAGE
Mahima Swamy and Wolfgang W.A. Schamel

Chapter 11: Non-Replicating Recombinant Vaccinia Virus Expressing CD80 to Enhance T-Cell Stimulation
Paul Zajac
This book is a collection of protocols, to provide novel techniques for the study of the biology of T lymphocytes. The methods described in this book do not cover all of the techniques currently used to study T cell-mediated immune responses for the simple reason that T cell immunology is probably the immunological discipline which can be investigated with the widest variety of approaches. Thechoiceofchapterswasmadetakingintoaccounttwopoints:First,manyofthe techniquesthathavebeen usedfor sometimehave beenupgraded duringthe pastfew years given the greater availability of a variety of products (i.e. cytokines, chemokines, monoclonal antibodies), of refined technical devices (i.e. novel cell culture and cell analysis equipments), and the development of novel instrumentation (i.e. multipa- metric flow cytometers, confocal microscopes). Therefore, in several chapters ''old techniques'', which remain fundamental to T cell immunology, are described in their ''modern'' versions. Secondly, the technical advancement has generated the possibility to establish novel assays to investigate T cell physiology. This is reflected in the chapters which describe the protocols that allow use of these modern approaches. The preparation of this book has required participation of several scientists, all leading experts in their respective fields. Without their enthusiastic participation, this work would not have been possible. Therefore, I thank all authors for their contri- tionsandacceptallcriticismformissingparts,orinformationordetails,forwhichonly I am responsible. I hope these protocols will be useful for young investigators who approach for the first time the complex field of immunology and for those more experienced scientists who look for concise and efficacious descriptions of novel methods.

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