Single Cell Diagnostics

Methods and Protocols
 Book
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ISBN-13:
9781588295781
Einband:
Book
Erscheinungsdatum:
15.06.2007
Seiten:
196
Autor:
Alan R. Thornhill
Gewicht:
499 g
Format:
238x159x22 mm
Sprache:
Englisch
Beschreibung:

Up-to-date, clinically applicable, validated protocols for single-cell diagnostics
Table of Contents
1. Non-invasive metabolic assessment of single cells
David K. Gardner

2. Laser Capture Microdissection for Analysis of Single Cells
Nobuki Nakamura, Katharina Ruebel, Long Jin, Xiang Qian, Heyu Zhang, and Ricardo V. Lloyd

3. Fluorescence in situ hybridization (FISH) on Single Cells (Sex Determination and Chromosome Rearrangements)
Paul N. Scriven and Caroline Mackie Ogilvie

4. Single cell PCR for PGD: methods, strategies and limitations
Karen Sermon and Martine De Rycke

5. Real-Time Quantitative PCR Measurement of Male Fetal DNA in maternal plasma
Bernhard Zimmermann, Ahmad El-Sheikhah, Kypros Nicolaides, Wolfgang Holzgreve, Sinuhe Hahn

6. Cell-Free Fetal DNA Plasma Extraction and Real Time-PCR Quantification
Jill L. Maron, Kirby L. Johnson, and Diana W. Bianchi

7. LATE-PCR and allied technologies: Real-time detection strategies for rapid, reliable diagnosis from single cells
Kenneth E. Pierce and Lawrence J. Wangh
8. Efficient isothermal amplification of the entire genome from single cells
Karen V. Schowalter, Jolene R. Fredrickson and Alan R Thornhill

9. Comparative Genomic Hybridization on single cells
Lucille Voullaire and Leeanda Wilton

10. Generation of amplified RNAs and cDNA libraries from single mammalian cells
James Adjaye

11. Real-time PCR to measure gene expression in single cells
Dagan Wells
12. Gender Determination and Detection of Aneuploidy in Single Cells Using DNA Array-Based Comparative Genomic Hybridisation (Array CGH)
Dong Gui Hui, Xin Yuan Guan, and Nicole Hussey

13. Microarray technology for mutation analysis of low template DNA samples
Chelsea Salvado and David Cram
This book applies modern molecular diagnostic techniques to the analysis of single cells, small numbers of cells, or cell extracts. Emphasis is placed on non-invasive analysis of single cell metabolites and the direct analysis of RNA and DNA from single cells, with a focus on polymerase chain reaction and fluorescence in situ hybridization. In particular, this handbook is essential for practitioners providing care for couples seeking treatment for infertility.

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