Quantum Dots

Applications in Biology
 Buch
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ISBN-13:
9781588295620
Einband:
Buch
Erscheinungsdatum:
01.05.2007
Seiten:
323
Autor:
Charles Z. Hotz
Gewicht:
595 g
Format:
246x164x25 mm
Serie:
374, Methods in Molecular Biology
Sprache:
Englisch
Beschreibung:

Table of Contents
Preface
Contributors
Part 1 - Imaging of fixed cells and tissues

1. Immunofluorescent Labeling of Proteins in Cultured Cells with Quantum Dot Secondary Antibody Conjugates
Richard L. Ornberg and Hongjian Liu

2. Immunohistochemical Detection with Quantum Dots
Rizwan S. Akhtar, Cecelia B. Latham, Dario Siniscalco, Carlo Fuccio and Kevin A. Roth

3. Quantum Dots for Multicolor Clinical Pathology and Multispectral Imaging
Johbu Itoh and Robert Yoshiyuki Osamura

4. Light and Electron Microscopic Localization of Multiple Proteins using Quantum dots
Thomas J. Deerinck, Ben N. G. Giepmans, Benjamin L. Smarr, Maryann E. Martone and Mark H. Ellisman

5. Human Metaphase Chromosome FISH using Quantum Dot Conjugates
Joan H.M. Knoll

Part 2 - Imaging of live cells

6. Biotin-Ligand Complexes with Streptavidin Quantum Dots for in vivo Cell Labeling of Membrane Receptors
Diane S. Lidke, Peter Nagy, Thomas M. Jovin and Donna J. Arndt-Jovin

7. Single Quantum Dot Tracking of Membrane Receptors
Cédric Bouzigues, Sabine Lévi, Antoine Triller and Maxime Dahan

8. Optical Monitoring of Single Cells Using Quantum Dots
Jyoti K. Jaiswal and Sanford M. Simon

9. Peptide Mediated Intracellular Delivery of Quantum Dots
B. Christoffer Lagerholm

10. High Content Analysis in Multiple Cell Lines Using Quantum Dots
Paul G. Wylie

11. Measuring Cell Motility Using Quantum Dot Probes
Weiwei Gu, Teresa Pellegrino, Wolfgang J. Parak, Rosanne Boudreau, Mark A. Le Gros, A. Paul Alivisatos and Carolyn A. Larabell

Part 3 - Imaging of live animals

12. Quantum Dots for In-vivo Molecular and Cellular Imaging
Xiaohu Gao, Leland WK Chung and Shuming Nie

13. Sentinel Lymph Node Mapping with Type II Quantum Dots
John V. Frangioni, Sang-WookKim, Shunsuke Ohnishi, Sungjee Kim and Moungi Bawendi

14. Macrophage-mediated Co-localization of Quantum Dots in Experimental Glioma
Osman Muhammad, Alexandra Popescu and Steven A. Toms

Part 4 - Flow cytometry applications

15. Applications of Quantum Dots to Multicolor Flow Cytometry
Pratip K. Chattopadhyay and Mario Roederer

16. Quantum Dots in Flow Cytometry
Barnaby Abrams and Tim Dubrovsky

Part 5 - Biochemical applications

17. Luminescent Biocompatible Quantum Dots: A tool for immunosorbent assay design
Ellen R. Goldman, H. Tetsuo Uyeda, Andrew Hayhurst, and Hedi Mattoussi

18. Fluorescence-based Analysis of Cellular Protein Lysate Arrays Using Quantum Dots
David H. Geho, J. Keith Killian, Animesh Nandi, Johanne Pastor, Prem Gurnani and Kevin P. Rosenblatt.

19. Application of Quantum Dots to Multi-Color Microarray Experiments: Four-Color Genotyping
George Karlin-Neumann, Marina Sedova, Mat Falkowski, Zhiyong Wang, Steven Lin and Maneesh Jain

Index
Quantum Dots captures many diverse applications enabling utility in biological detection. Organized into five parts, the first two parts cover the use of QDs in imaging fixed and living cells (and tissues). Protocols are included for using QDs in routine as well as enabling applications. Part 3 shows early efforts aimed at using QDs in live animals. The final two parts demonstrate the versatility of QD technology in existing assay technology.

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