In Vitro Transcription and Translation Protocols

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ISBN-13:
9781588295583
Einband:
Buch
Erscheinungsdatum:
01.06.2007
Seiten:
276
Autor:
Guido Grandi
Gewicht:
584 g
Format:
236x159x27 mm
Serie:
375, Methods in Molecular Biology
Sprache:
Englisch
Beschreibung:

This book is a highly anticipated update of the previous edition. It provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. It has been completely updated with new chapters and topics.
This book is a highly anticipated update of the previous edition. In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.
Section One: TECHNOLOGIES
Chapter One - Energy Systems for ATP Regeneration in Cell-free Protein Synthesis Reactions
Kara A. Calhoun, James R. Swartz

Chapter 2 - Continuous-exchange Protein-synthesizing Systems
Vladimir A. Shirokov, Aigar Kommer, Vyacheslav A. Kolb, Alexander S. Spirin

Chapter 3 - Cell-free production of integral membrane proteins on a preparative scale'
Christian Klammt, Daniel Schwarz, Vilker Dötsch, Frank Bernhard

Chapter 4- SIMPLEX: Single-molecule PCR-linked in vitro expression: a novel method for high-throughput construction and screening of protein libraries
Suang Rungpragayphan, Tsuneo Yamane, Hideo Nakano

Chapter 5 - Methods for High-throughput Materialization of Genetic Information Based on Wheat Germ Cell-free Expression System
Tatsuya Sawasaki, Ryo Morishita, Mudeppa D. Gouda, Yaeta Endo

Chapter 6 - Exogenous Protein Expression in Xenopus Oocytes: Basic Procedures
Elena Bossi, Maria Serena Fabbrini, Aldo Ceriotti

Section Two: APPLICATIONS

Chapter 7 -In vitro translation to study HIV protease activity
Zene Matsuda, Mutsunori Iga, Kosuke Miyauchi, Jun Komano, Kazuhiro Morishita, Akihiko Okayama, Hirohito Tsubouchi

Chapter 8 - The protein truncation test in mutation detection and molecular diagnosis
Oliver Hauss, Oliver Müller

Chapter 9 - Creation of Novel Enantioselective lipases by SIMPLEX
Yuichi Koga, Tsuneo Yamane, Hideo Nakano

Chapter 10 - In vitro transcription and translation coupled to 2D-electrophoresis for bacterial proteome analysis
Nathalie Norais, Ignazio Garaguso, Germano Ferrari, Guido Grandi

Chapter 11 - In vitro Screen of Bioinformatically Selected Bacillus anthracis Vaccine Candidates by Coupled Transcription, Translation and Immunoprecipitation Analysis
Orit Gat, Haim Grosfeld,Avigdor Shafferman

Chapter 12 - Functional expression of type 1 rat GABA transporter in microinjected Xenopus laevis oocytes
Stefano Giovannardi, Simona Magagnin, Andrea Soragna, Laura Faravelli

Chapter 13 - Production of Protein for NMR Study Using the Wheat Germ Cell-Free System
Toshiyuki Kohno, Yaeta Endo
A highly anticipated update of the previous edition, In Vitro Transcription and Translation Protocols, Second Edition, provides molecular biology laboratories with the most powerful techniques for exploiting in vitro transcription and translation systems. Completely updated with new chapters and topics, there is in-depth analysis of current technologies and applications of in-vitro transcriptions and translations systems. Detailed protocols for protein production using different in vitro transcription and translation systems are included.

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