A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Includes supplementary material: sn.pub/extras

An Introduction to Plant Cell Culture.- History of Plant Tissue Culture.- Cell Culture and Plant Regeneration: The Fundamentals.- Pathogen and Biological Contamination Management.- Growth Measurements.- Callus and Suspension Culture Induction, Maintenance, and Characterization.- Measurement of Cell Viability in In Vitro Cultures.- Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification.- Somatic Embryogenesis in Picea Suspension Cultures.- Indirect Somatic Embryogenesis in Cassava for Genetic Modification Purposes.- Direct Somatic Embryogenesis in Coffea canephora.- PLANT PROPAGATION IN VITRO.- A New Temporary Immersion Bioreactor System for Micropropagation.- Protocol to Achieve Photoautotrophic Coconut Plants Cultured In Vitro With Improved Performance Ex Vitro.- Use of Statistics in Plant Biotechnology.- An Efficient Method for the Micropropagation of Agave Species.- Micropropagation of Endangered Plant Species.- Clonal Propagation of Softwoods.- Applications for Plant Protoplasts.- Isolation, Culture, and Plant Regeneration From Leaf Protoplasts of Passiflora.- Isolation, Culture, and Plant Regeneration From Echinacea purpurea Protoplasts.- Production of Cybrids in Brassicaceae Species.- Guard Cell Protoplasts.- Production of Interspecific Hybrid Plants in Primula.- Protocols for Genomic Manipulation.- Agrobacterium-Mediated Transformation of Petunia Leaf Discs.- Transformation of Wheat Via Particle Bombardment.- Chloroplast Transformation.- The Biochemical Basis for the Resistance to Aluminum and Their Potential as Selection Markers.- Transformation of Maize Via Agrobacterium tumefaciens Using a Binary Co-Integrate Vector System.- Accumulation of Metabolites in Plant Cells.- Capsaicin Accumulation in Capsicum spp. Suspension Cultures.- Isolation and Purification of Ribosome-Inactivating Proteins.- Catharanthus roseus Shoot Cultures for the Production of Monoterpenoid Indole Alkaloids.- Methods for Regeneration and Transformation in Eschscholzia californica.