Pichia Protocols

 Book
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ISBN-13:
9781588294296
Einband:
Book
Erscheinungsdatum:
08.08.2007
Seiten:
280
Autor:
James M. Cregg
Gewicht:
558 g
Format:
229x152x21 mm
Sprache:
Englisch
Beschreibung:

This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication.
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. The volume fully updates and expands upon the first edition, and focuses primarily on information that has come to light since its original publication. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI).
Table of Contents
Chapter 1 - Introduction [REVIEW CHAPTER]

Chapter 2 - Vectors and strains for expression [REVIEW CHAPTER]

Chapter 3 - DNA-Mediated Transformation

Chapter 4 - Rational design and optimization of fed-batch and continuous fermentations

Chapter 5 - Overexpression of a hookworm secretory protein saturates secretory capacity

Chapter 6 - Initial processing and purification strategies

Chapter 7 - Rapid screening of chromatography resins for the purification of proteins

Chapter 8 - Characterization of O-linked saccharides on glycoproteins

Chapter 9 - Modification of the N-glycosylation pathway to produce homogeneous human-like glycans using GlycoSwitch plasmids

Chapter 10 - N-linked glycan characterization of heterologous proteins

Chapter 11 - Heavy labeling of recombinant proteins

Chapter 12 - Selenomethionine labeling of recombinant proteins

Chapter 13 - Deuterium labeling of proteins

Chapter 14 - Classical genetic analysis

Chapter 15 - Identification of pexophagy genes by Restriction Enzyme-Mediated Integration (REMI)

Chapter 16 - Characterization of protein-protein interactions

Chapter 17 - Localization of proteins and organelles using fluorescence microscopy

Chapter 18 - Fluorescence microscopy and thin-section electron microscopy
This book focuses on recent developments of Pichia pastoris as a recombinant protein production system. Highlighted topics include a discussion on the use of fermentors to grow Pichia pastoris, information on the O- and N-linked glycosylation, methods for labeling Pichia pastoris expressed proteins for structural studies, and the introduction of mutations in Pichia pastoris genes by the methods of restriction enzyme-mediated integration (REMI). Each chapter presents cutting-edge and cornerstone protocols for utilizing P. pastoris as a model recomibinant protein production system. This volume fully updates and expands upon the first edition.

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