This second edition volume expands on the previous edition with a comprehensive update on recent developments in the Adipose Stromal/Stem Cells (ASC) field and the use of stroma vascular fraction (SVF) as a potential therapeutic strategy. The chapters in this book discuss protocols that are focused on discovery, preclinical, and clinical processes. Although most chapters focus on human ASC, there are several chapters that look at small and large animal species with pre-clinical relevance. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips in troubleshooting and avoiding known pitfalls.

Includes cutting-edge methods and protocols

Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose Tissue.- A Method for Isolation of Stromal Vascular Fraction Cells in a Clinically Relevant Timeframe.- Scaffold-Free, Size-Controlled Three-Dimensional Culture of Rabbit Adipose-Derived Stem Cells.- The Differentiation of Brown Adipocyte Progenitors Derived from Human Induced Pluripotent Stem Cells.- Methylcellulose Based Thermally Reversible Hydrogels.- Decellularized Adipose Tissue Scaffolds for Soft Tissue Regeneration and Adipose-Derived Stem/Stromal Cell Delivery.- Induction of Skin Allograft Transplantation Tolerance in Mice Using Human Adipose Derived Stromal Cells.- High Definition Confocal Imaging Modalities for the Characterization of Tissue-Engineered Substitutes.- Adipose-Derived Stromal Vascular Fraction Cells and Platelet-Rich Plasma: Basic and Clinical Implications for Tissue Engineering Therapies in Regenerative Surgery.- Myogenic Differentiation of ASCs using Biochemical and Biophysical Induction.- Isolation of Murine Adipose-Derived Stromal/Stem Cells for Adipogenic Differentiation or Flow Cytometry-Based Analysis.- Three-Dimensional Magnetic Levitation Culture System Simulating White Adipose Tissue.- Isolation of Human Adipose-Derived Stem Cells from Lipoaspirates.- Isolation of Murine Adipose-Derived Stromal/Stem Cells using an Explant Culture Method.- Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation.- Soft Tissue Reconstruction.- Mechanical Stimulation of Adipose-Derived Stem Cells for Functional Tissue Engineering of the Musculoskeletal System via Cyclic Hydrostatic Pressure, Simulated Microgravity, and Cyclic Tensile Strain.- Cryopreservation Protocols for Human Adipose Tissue Derived Adult Stem Cells.- Bone Regeneration with a Combination of Adipose-Derived Stem Cells and Platelet-Rich Plasma.- Isolationand Differentiation of Murine Primary Brown/Beige Preadipocytes.