This volume presents a compilation of various representative techniques and approaches currently used to study bacterial foodborne pathogens. Chapters guide the reader through bacterial pathogen detection and quantification in food, molecular, phenotypic, metabolic characterization of food pathogens, and ecology of foodborne bacterial pathogens. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Foodborne Bacterial Pathogens : Methods and Protocols aims to server as a guide both for researchers, students, and those in the food industry who want to have an overview of current approaches and protocols used to study bacterial foodborne pathogens.

Includes cutting-edge method and protocols

Accelerated Sample Preparation for Fast Salmonella Detection in Poultry Products.- Direct or DNA Extraction-free Amplification and Quantification of Foodborne Pathogens.- The Use of Multiplex Real Time PCR for the Simultaneous Detection of Food-borne Bacterial Pathogens.- Sequence Specific End Labelling of Oligonucleotides (SSELO)-based Microbial Detection.- Rapid on-site Detection and Quantification of Food-borne Pathogens Using Microfluidic Devices.- Modified Bacteriophage Tail Fiber Proteins for Labeling, Immobilization, Capture, and Detection of Bacteria.- EIS-based Biosensors in Foodborne Pathogen Detection with a Special Focus on Listeria monocytogenes.- Method to Study the Survival Abilities of Foodborne Bacterial Pathogens under Food Processing Conditions.- Viability Detection of Foodborne Bacterial Pathogens in Food Environment by PMA-qPCR and by Microscopy Observation.- Isolation of Bacterial RNA from Foods Inoculated with Pathogens.- The use of Two-dimensional Electrophoresis to Explore Foodborne Bacterial Pathogens Responses to Gastrointestinal Stress.- Identification of Putative Biomarkers Specific to Food-borne Pathogens Using Metabolomics.- Characterization of Bacterial Membrane Fatty Acid Profiles for Biofilm Cells.- High Content Screening Confocal Laser Microscopy (HCS-CLM) to Characterize Biofilm 4D Stuctural Dynamic of Food-borne Pathogens.- Static Immersion and Injection Methods for Live Cell Imaging of Foodborne Pathogen Infections in Zebrafish Larvae.- Use of C. elegans Diapause to Study Transgenerational Responses to Pathogen Infection.- Utilizing the public GenomeTrakr Database for Foodborne Pathogen Traceback.- The Establishment of a Standarized 16srDNA Library Preparation to Enable the Analysis of the Microbiome in Poultry Processing Using the Illumina MiSeq Platform.- Exploring Foodborne Pathogen Ecology and Antimicrobial Resistance in the Light of Shotgun Metagenomics.- Modeling Growth of Listeria and Lactic Acid Bacteria in Food Environments.- Network Biology Approaches to Identify Molecular and Systems-level Differences between Salmonella Pathovars.