This volume discusses certain epigenetic changes recognized in early carcinogenic lesions and different tumors, as well as factors that alter the epigenome and epigenetic profile such as diet, alcohol, immunity, circadian rhythm, and more. The chapters in this book further delve into this field and cover topics such as epigenome-based precision medicine in lung cancer; interplay between genetic and epigenetic changes in breast cancer subtypes; genetic regulation of PDCD1 gene in cancer immunology; and pyrosequencing methylation analysis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Early Epigenetic Markers for Precision Medicine.- Interplay between Genetic and Epigenetic Changes in Breast Cancer Subtypes.- Role of Microbiome in Carcinogenesis Process and Epigenetic Regulation of Colorectal Cancer.- Epigenome-Based Precision Medicine in Lung Cancer.- Epigenetics in Hematological Malignancies.- MicroRNAs Role in Prostate Cancer.- The Effects of Dietary Nutrients on Epigenetic Changes in Cancer.- Diet, Microbiome, and Epigenetics in the Era of Precision Medicine.- Alcohol-Induced Epigenetic Changes in Cancer.- Epigenetic Basis of Circadian Rhythm Disruption in Cancer.- Epigenetic Changes of the Immune System with Role in Tumor Development.- DNA Methylation as a Biomarker of Aging in Epidemiologic Studies.- Challenges and Opportunities in Social Epigenomics and Cancer.- Epigenetic and Genetic Regulation of PDCD1 Gene in Cancer Immunology.- Methylation and Micro RNA Profiling to Understand Racial Disparities of Prostate Cancer.- Analysis of DNA Hypermethylation in Pancreatic Cancer using Methylation Specific PCR and Bisulfite Sequencing.- Pyrosequencing Methylation Analysis.