This volume discusses the latest tools, techniques, and animal models designed to study the processes of lymphatic vascular formation in vivo and in vitro and its functions in health and disease. The chapters in the book cover topics such as genetics lineage tracing of lymphatic endothelial cells in mice; characterization of zebrafish facial lymphatics; imaging lymphatics in mouse lungs; effects of fluid shear stress of lymphatic endothelial cells; and single cell mRNA sequencing of the mouse brain vasculature. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, Lymphangiogenesis: Methods and Protocols is a valuable resource to aid researchers with applying new approaches to answer their questions in this developing field.

Includes cutting-edge methods and protocols

Three-Dimensional Visualization of the Lymphatic Vasculature.- The Histological and Morphological Characterization of Developing Dermal Lymphatic Vessels.- Genetic Lineage Tracing of Lymphatic Endothelial Cells in Mice.- Visualization and Tools for Analysis of Zebrafish Lymphatic Development.- Characterization of Zebrafish Facial Lymphatics.- Correlative Fluorescence and Scanning Electron Microscopy to Study Lymphovenous Valve Development.- Characterization of Mouse Mesenteric Lymphatic Valve Structure and Function.- Morphological Analysis of Lacteal Structure in the Small Intestine of Adult Mice.- Morphological and Functional Analysis of CNS Associated Lymphatics.- Morphological Analysis of Schlemm's Canal in Mice.- Imaging Lymphatics in Mouse Lungs.- Imaging of Endothelial Cell Dynamic Behavior in Zebrafish.- Visualization and Measurement of Lymphatic Function In Vivo.- Investigating Effects of Fluid Shear Stress on Lymphatic Endothelial Cells.- Methods for Assessing the Contractile Function of Mouse Lymphatic Vessels Ex Vivo.- Evaluation and Characterization of Endothelial Cell Invasion and Sprouting Behavior.- Dorsal Ear Skin Window for Intravital Imaging and Functional Analysis of Lymphangiogenesis.- Isolation of Human Skin Lymphatic Endothelial Cells and 3D Reconstruction of the Lymphatic Vasculature In Vitro.- Stimulation and Inhibition of Lymphangiogenesis via Adeno-Associated Viral Gene Delivery.- Isolation and Characterization of Mouse Organ Specific Endothelial Transcriptomes.- Single Cell mRNA Sequencing of the Mouse Brain Vasculature.- Metabolic Analysis of Lymphatic Endothelial Cells.- Characterizing Epigenetic Changes in Endothelial Cells.