This detailed book provides general protocols and technologies that plant biologists worldwide often utilize for the purpose of accelerating research progress in the field of plant transcription factors. Beginning with a brief introduction, the volume continues by exploring methods in the preparation of plant materials, detection of expression levels, interaction tests, and chromatin analyses. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Includes cutting-edge methods and protocols for the study of plant transcription factors

Master Regulatory Transcription Factors in Plant Development: A Blooming Perspective.- Application of CRISPR/Cas to Understand Cis- and Trans-Regulatory Elements in Plants.- The Long-Term 'In Natura' Study Sites of Arabidopsis halleri for Plant Transcription and Epigenetic Modification Analyses in Natural Environments.- Generation of Inducible Transgenic Lines of Arabidopsis Transcription Factors Regulated by MicroRNAs.- A Specific Knockdown of Transcription Factor Activities in Arabidopsis.- Using CRISPR/Cas9 System to Introduce Targeted Mutation in Arabidopsis.- CRISPR/Cas9-Based Genome Editing of Transcription Factor Genes in Marchantia polymorpha.- Cell-Type-Specific Promoter Identification Using Enhancer Trap Lines.- Isolation of Arabidopsis Palisade and Spongy Mesophyll Cells.- Ectopic Vascular Induction in Arabidopsis Cotyledons for Sequential Analysis of Phloem Differentiation.- High Impact Gene Discovery: Simple Strand Specific mRNA Library Construction and Differential Regulatory Analysis Based on Gene Co-Expression Network.- Laser Capture Micro-Dissection Coupled to RNA Sequencing: A Powerful Approach Applied to the Model Legume Medicago truncatula in Interaction with Sinorhizobium meliloti.- nanoCAGE-XL: An Approach to High-Confidence Transcription Start Site Sequencing.- Genome-Wide TSS Identification in Maize.- Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee.- Two-Color In Situ Hybridization: A Technique for Simultaneous Detection of Transcripts from Different Loci.- Gene Expression and Transcription Factor Binding Tests Using Mutated-Promoter Reporter Lines.- Rapid and Quantitative CELD Assay to Measure the Specificity of Transcription Factor-DNA Binding Interactions and Identify cis-Elements.- In Situ Proximity Ligation Assay to Detect the Interaction between Plant Transcription Factors and Other Regulatory Proteins.- Cell-Free Protein Synthesis of PlantTranscription Factors.- Application of MNase-Seq in the Global Mapping of Nucleosome Positioning in Plants.- Genome-Wide Mapping of DNase I Hypersensitive Sites in Tomato.- Genome-Wide Identification of Chromatin Domains Anchored at the Nuclear Periphery in Plants.