This detailed volume focuses on the development of mycosis vaccines via the most common etiological agents of mycoses, such as Aspergillus and Candida species, followed by Cryptococcus, dimorphic fungi, and members of Mucoraceae family of fungi. The book is sectioned into five parts that describe experimental approaches for vaccine candidate discovery, improved vaccine design and delivery, assessment of critical endpoints, analysis of mechanisms of mycosis vaccine protection, and key methods for clinical translation. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting known pitfalls.

Includes cutting-edge techniques for the study of invasive mycosis vaccine creation and implementation

Mouse Immunization with Radioattenuated Yeast Cells ofParacoccidioides brasiliensis.Heat-Killed Yeast as a Pan-Fungal Vaccine.- Immunoinformatics as a Tool for New Antifungal Vaccines.- Rational Design of T Lymphocyte Epitope-Based Vaccines AgainstCoccidioidesInfection.- Identification of Fungal T Cell Epitopes by Mass Spectrometry-Based Proteomics.- Intranasal Antifungal Vaccination Using DNA-Transfected Dendritic Cells.- DNAhsp65 Vaccine as Therapy Against Paracoccidioidomycosis.- Idiotypic Antifungal Vaccination: Immunoprotection by Antiidiotypic Antibiotic Antibodies.- Peptide Vaccine Against Paracoccidioidomycosis.- Methodology for Anti-Cryptococcal Vaccine Development.- Beta-Glucan Particles as Vaccine Adjuvant Carriers.- Th1-Inducing Agents in Prophylaxis and Therapy for Paracoccidioidomycosis.- Nanoparticle-Based Mycosis Vaccine.- Yeast Expressing Gp43 Protein as a Vaccine AgainstParacoccidioides brasiliensisInfection.- Vaccination with Phage-Displayed Antigenic Epitope.- Preparation of an Oral Vaccine by Proteome Analysis and Molecular Display Technology.- Precise and Efficient In-Frame Integration of an Exogenous GFP Tag inAspergillus fumigatusby a CRISPR System.- Endpoint Assessment in Rabbit Models of Invasive Pulmonary Aspergillosis and Mucormycosis.- CD4+T Cells Mediate Aspergillosis Vaccine Protection.- T-Cell-Mediated Cross-Protective Immunity.- Assessment of Post-Vaccination Phagocytic Activation UsingCandida albicansKilling Assays.- Immunization with Antigen-Pulsed Dendritic Cells Against Highly VirulentCryptococcus gattiiInfection: Analysis of Cytokine-Producing T Cells.- Testing Antifungal Vaccines in an Animal Model of Invasive Candidiasis and in Human Mucosal Candidiasis.