This volume provides detailed discussion of a variety of important techniques that researchers use to study fungal molecular biology and pathogenesis. Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Candida Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

Includes cutting-edge methods and protocols

PART I: Molecular Analyses Of Pathogenesis  1 How to use the Candida Genome Database Marek S. Skrzypek, Jonathan Binkley, and Gavin Sherlock 2 Transcriptional Profiling of Candida albicans in the Host Kearney T.W. Gunsalus, Carol A. Kumamoto 3 SRYTH: A New Yeast Two-Hybrid Method Jaideep Mallick, Gregor Jansen, Cunle Wu and Malcolm Whiteway 4 Chromatin immunoprecipitation (ChIP) assay in Candida albicans Sreyoshi Mitra,  Laxmi Shanker Rai,  Gautam Chatterjee,  and Kaustuv Sanyal 5 Assessing mitochondrial functions in Candida albicansRichard Calderone and Dongmei Li 6  Imaging Candida Infections in the HostDhammika H. Navarathn, David D. Roberts, Jeeva Munasinghe,  and Martin J. Lizak  7 Identification of Secreted Candida Proteins using Mass Spectrometry Emilia Gómez-Molero, Henk L. Dekker, Albert D. de Boer,  and Piet W.J. de Groot 8 In vitro models for Candida Biofilm Development Bastiaan P. Krom and Hubertine M.E. Willems 9 The Fungal Biome of the Oral Cavity  Jyotsna Chandra, Mauricio Retuerto, Pranab K. Mukherjee, and Mahmoud Ghannoum 10  Experimental models of C. albicans-streptococcal Co-infectionTakanori Sobue, Patricia Diaz, Hongbin Xu, Martinna Bertolini, and Anna Dongari-Bagtzoglou 11  Staphylococcus-Candida interaction models: Antibiotic Resistance Testing and Host InteractionNina Scheres and Bastiaan P. Krom  PART II:  Drug Discovery and Susceptibility Testing 12  Genetic Screens for Determination of Mechanism of Action  Françoise Gay-Andrieu, Deepu Alex, and Richard Calderone 13 Microbroth Dilution Susceptibility Testing of Candida Species  Randal J. Kuykendall and Shawn R. Lockhart 14 Biofilms and Antifungal Susceptibility Testing Maria Simitsopoulou, Athanasios Chatzimoschou, and Emmanuel Roilides15  Typing Candida Species using Microsatellite Length Polymorphism and Multilocus Sequence TypingDea Garcia-Hermoso, Marie Desnos-Ollivier, and Stéphane Bretagne  16   Diagnostic Methods for Detection of Blood-borne CandidiasisCornelius J. Clancy, and M. Hong Nguyen