Biochemical Approaches for Glutamatergic Neurotransmission

130, Neuromethods
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Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible results
Contains key notes and implementation advice from the experts
Series Preface...
Preface...
Table of Contents...
Contributing Authors...

1. Identification of Glutamatergic Neurons
Shiliang Zhang and Marisela Morales

2. Single Nanoparticle Tracking: A Method for Investigating the Surface Dynamics of Glutamate Receptors
Julien Dupuis, François Maingret, and Laurent Groc

3. Positron Emission Tomography of Metabotropic Glutamate Receptors
Selena Milicevic Sephton

4. NMR Spectroscopy of Brain Glutamate Function
Jun Shen

5. Imaging Glutamate with Genetically Encoded Fluorescent Sensors
Gerard J. Broussard, Elizabeth K. Unger, Ruqiang Liang, Brian P. McGrew, and Lin Tian

6. Measurement of Astrocytic Glutamate Release using Genetically-Encoded Probe Combined with TIRF Microscopy
Fuyuko Takata-Tsuji, Naura Choulnamountri, Christophe Place, and Olivier Pascual
7. Drugs to Alter Extracellular Concentration of Glutamate: Modulators of Glutamate Uptake Systems
Andréia Cristina Karklin Fontana
This volume presents techniques and recent developments in biochemical approaches to study glutamatergic neurotransmission. This book contains detailed discussions on tracing neuronal pathways, functional or spectroscopic imaging, optogenetic or pharmacological tools, and extracellular neurochemistry in experimental clinical models. The chapters cover topics such as: identification of glutamatergic neurons in a discrete brain area, tools to study dynamics of single ionotropic receptors, in vivo extracellular glutamate monitoring in preclinical research using biosensors, capillary electrophoresis and liquid chromatography, and potential brain glutamatergic clinical biomarkers in biofluids and tissues. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.

Cutting-edge and authoritative, Biochemical Approaches for Glutamatergic Neurotransmission is a valuable resource for researchers studying glutamate neurochemistry.

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Autor: Sandrine Parrot
ISBN-13 :: 9781493972272
ISBN: 1493972278
Erscheinungsjahr: 01.10.2017
Verlag: Springer-Verlag GmbH
Gewicht: 1276g
Seiten: 565
Sprache: Englisch
Auflage 2017
Sonstiges: Buch, 261x185x38 mm, 37 schwarz-weiße und 105 farbige Abbildungen, 105 farbige Tabellen, Bibliographie
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