RNA Detection

1649, Methods in Molecular Biology
Methods and Protocols
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Includes cutting-edge methods and protocols
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Preface... Table of Contents...
Contributing Authors...
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This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

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Autor: Imre Gaspar
ISBN-13 :: 9781493972128
ISBN: 149397212X
Erscheinungsjahr: 01.02.2018
Verlag: Springer-Verlag GmbH
Gewicht: 1123g
Seiten: 492
Sprache: Englisch
Sonstiges: Buch, 261x179x35 mm, 15 schwarz-weiße und 79 farbige Abbildungen, Bibliographie
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