Proteomics for Drug Discovery

1647, Methods in Molecular Biology
Methods and Protocols
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Includes cutting-edge techniques for the study of drug discovery via proteomics
1. A Photoaffinity Labeling-Based Chemoproteomics Strategy for Unbiased Target Deconvolution of Small Molecule Drug Candidates
This book reflects the drug development community's interest in proteomics methods by providing researchers with the necessary knowledge for the implementation of a number of basic experimental and bioinformatics protocols in the drug discovery research laboratory. The focus of the collection is on select protocols and methodologies that address the analysis of post-translational modifications, targeted protein quantification, protein-protein, protein-lipid, or protein-ATP interactions, in silico lead identification, protein arrays, tissue and cell extract preparation, labeling, chemoproteomics, and drug efficacy assessment. Emphasis was placed not just on methods that describe large-scale protein analysis, but also on ones that are amenable to future implementation in a high-throughput format. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

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Autor: Iuliana M. Lazar
ISBN-13 :: 9781493972005
ISBN: 1493972006
Erscheinungsjahr: 05.09.2017
Verlag: Springer-Verlag GmbH
Gewicht: 784g
Seiten: 302
Sprache: Englisch
Sonstiges: Buch, 261x184x25 mm, 17 schwarz-weiße und 49 farbige Abbildungen, Bibliographie
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