Plant Argonaute Proteins

1640, Methods in Molecular Biology
Methods and Protocols
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Includes cutting-edge methods and protocolsProvides step-by-step essential for reproducible resultsContains key notes and implementation advice from the expert
1. Plant ARGONAUTEs: Features, Functions and Unknowns
This volume provides the reader with step-by-step methods to study plant ARGONAUTE (AGO) functions. Chapters in Plant Argonaute Poteins: Methods and Protocols summarize the main biochemical, molecular and cytological methods to study AGO-small RNA complexes and their interactions with target RNAs, AGO subcellular localization, AGO association with polysomes, AGO role in meiosis and DNA repair, methods for the identification, cloning and characterization of AGO genes in different plant species, and non-radioactive protocols for small RNA detection. Finally, a series of chapters describing computational methods to study plant AGO function and evolution are provided. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Argonaute Proteins: Methods and Protocols aims to deliver the most complete and updated list of protocols to study plant AGO function.

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Autor: Alberto Carbonell
ISBN-13 :: 9781493971640
ISBN: 1493971646
Erscheinungsjahr: 01.06.2017
Verlag: Springer-Verlag GmbH
Gewicht: 766g
Seiten: 297
Sprache: Englisch
Sonstiges: Buch, 261x181x25 mm, 22 schwarz-weiße und 25 farbige Abbildungen, Bibliographie
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