Circulating Tumor Cells

1634, Methods in Molecular Biology
Methods and Protocols
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Includes cutting-edge methods and protocols
ating Tumor Cells for RT-PCR Analysis
Priya Gogoi, Saedeh Sepehri, Will Chow, Kalyan Handique, and Yixin Wang
This volume explores various approaches for enrichment, detection, isolation, and molecular profiling of circulating tumor cells (CTCs). Each chapter provides comprehensive descriptions and guidelines on how to perform innovative experiments in CTC research. Included are protocols for capture of CTCs via filtration and density gradient centrifugation; microfluidic and immunomagnetic separation of CTCs; detection of CTCs by immunocytochemistry, fluorescence in situ hybridization, and flow cytometry; assays designed for genomic characterization and functional analyses of CTCs, and many more. Written in the highly successful Methods in Molecular Biology series format, the chapters in this book include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and authoritative, Circulating Tumor Cells: Methods and Protocols is a valuable resource for laboratory researchers and clinicians who are interested in furthering their studies on CTCs.

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Autor: Mark Jesus Magbanua
ISBN-13 :: 9781493971435
ISBN: 1493971433
Erscheinungsjahr: 18.08.2017
Verlag: Springer-Verlag GmbH
Gewicht: 808g
Seiten: 306
Sprache: Englisch
Sonstiges: Buch, 264x179x25 mm, 10 schwarz-weiße und 76 farbige Abbildungen, Bibliographie
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