Fibrosis

1627, Methods in Molecular Biology
Methods and Protocols
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Includes cutting-edge methods and protocols
Part II: Animal Models of Fibrosis 2. The Bleomycin Model of Pulmonary Fibrosis
Tianju Liu, Francina Gonzalez De Los Santos, and Sem H. Phan
This volume describes state-of-the-art protocols that serve as "recipes" for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis in the lungs, skin, liver and heart, and generating transgenic mouse models. Part II discusses cell culture systems, where the chapters explore cell types important for the development of fibrosis. Part III looks at the purification, quantification, and analysis of the ECM proteins, and Part IV describes computer-assisted methods such as quantifying fibrillar collagen alignment and exploring the nano-surface of collagen with atomic force microscopy (AFM). Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Fibrosis: Methods and Protocols is a valuable resource aimed at outstanding quality and repeatability of research experiments in the fibrosis field.

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Autor: Laure Rittie
ISBN-13 :: 9781493971121
ISBN: 1493971123
Erscheinungsjahr: 01.09.2017
Verlag: Springer-Verlag GmbH
Gewicht: 1147g
Seiten: 530
Sprache: Englisch
Sonstiges: Buch, 259x179x35 mm, 30 schwarz-weiße Abbildungen, Bibliographie
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