Cell culture methodologies have become standard procedures in most plant laboratories. Currently,  facilities for in vitro cell cultures are found in practically every plant biology laboratory, serving different purposes since tissue culture has turned into a basic asset for modern biotechnology, from the fundamental biochemical aspects to the massive propagation of selected individuals. ¿Plant Cell Culture Protocols, Third Edition is divided into five convenient sections that cover topics from general methodologies, such as culture induction, growth and viability evaluation, statistical analysis and contamination control, to highly specialized techniques, such as clonal propagation, haploid production, somatic embryogenesis, organelle transformation. The volume concludes with a section on the laborious process of measuring the epigenetics changes in tissue cultures.¿Written in the successful Methods in Molecular Biology¿ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Plant Cell Culture Protocols, Third Edition seeks to serve both professionals and novices with its guide to the most common and applicable techniques and methods for plant tissue and cell culture.

Includes cutting-edge methods and protocols

1. An Introduction to Plant Cell Culture                Victor M. Loyola-Vargas and Neftalí Ochoa-Alejo 2. History of Plant Tissue Culture                Trevor Thorpe 3. Callus, Suspension Culture and Hairy Roots. Induction, Maintenance, and Characterization                Rosa M. Galáz-Ávalos, Sagrario Aguilar-Díaz, Pedro A. Xool-González, Silvia M. Huchín-May and Víctor M. Loyola-Vargas  4. Growth Measurements. Estimation of Cell Division and Cell Expansion                Gregorio Godoy-Hernández and Felipe A. Vázquez Flota 5. Measurement of Cell Viability                Lizbeth A. Castro-Concha, Rosa María Escobedo, and María de Lourdes Miranda-Ham 6. Pathogen and Biological Contamination Management in Plant Tissue Culture: Phyto-Pathogens, Vitro-Pathogens, and Vitro-Pests                Alan C. Cassells 7. Cryopreservation of Embryogenic Cell Suspensions by Encapsulation-Vitrification and Encapsulation-Dehydration                Zhenfang Yin, Long Chen, Bing Zhao, Yongxing Zhu, and Qiaochun Wang 8. The Study of in vitro Development in Plants: General Approaches and Photography                Edward C. Yeung 9. Use of Statistics in Plant Biotechnology                Michael E. Compton 10. Tissue Culture Methods for the Clonal Propagation and Genetic Improvement of Spanish Red Cedar (Cedrela Odorata)                Yuri Peña-Rodríguez,  Juan  Juárez Gómez, José Antonio  González, and  Manuel L. Robert 11. Micropropagation of Banana                Y¿ld¿z Aka Kaçar and Ben Faber 12. Liquid in vitro Culture for the Propagation of Arundo donax                Miguel Ángel Herrera-Alamillo and Manuel L. Robert 13. Production of Haploids and Doubled Haploids in Maize                Vanessa Prigge and Albrecht E. Melchinger 14. Maize Somatic Embryogenesis: Recent Features to Improve Plant Regeneration                Verónica Garrocho-Villegas, Maria Teresa de Jesús-Olivera, and Estela Sánchez Quintanar 15. Improved Shoot Regeneration from Root Explants Using an Abscisic Acid Containing Medium                Subramanian Paulraj and Edward C. Yeung 16. Cryopreservation of Shoot Tips and Meristems: An Overview of Contemporary Methodologies                Erica E. Benson and Keith Harding 17. Anther Culture of Chili Pepper (Capsicum spp.)                Neftalí Ochoa-Alejo 18. Production of Inter-Specific Hybrids in Ornamental Plants                Juntaro Kato and Masahiro Mii 19. Plant Tissue Culture of Fast-Growing Trees for Phytoremediation Research                José Luis Couselo, Elena Corredoira, Ana M. Vieitez, and Antonio Ballester         20. Removing Heavy Metals by in vitro Cultures                 María del Socorro Santos-Díaz and María del Carmen Barrón-Cruz 21. Establishment of a Sanguinarine Producing Cell Suspension Culture of Argemone Mexicana  L. (Papaveraceae). Induction of  Alkaloid Accumulation                Felipe A. Vázquez-Flota, Miriam Monforte-González, Cecilia Guízar-González, Jorge Rubio-Piña, and Karen Trujillo-Villanueva 22. Epigenetics, the Role of DNA Methylation on Tree Development                Marcos Viejo Somoano, María E. Santamaría, José L. Rodríguez, Luis Valledor, Mónica Meijón, Marta Pérez, Jesús Pascual, Rodrigo Hasbún, Mario Fernández Fraga, María Berdasco, Peter E. Toorop, María J. Cañal, and Roberto Rodríguez Fernández 23. The Potential Roles of microRNAs in Molecular Breeding                Qing Liu and Yue-Qin Chen 24. Determination of Histone Methylation in Mono- and Dicotyledons Plants                Geovanny I. Nic-Can and Clelia De la Peña 25. Basic Procedures for Epigenetic Analysis in Plant Cell and Tissue Culture                José L. Rodríguez, Jesús Pascual, Marcos Viejo Somoano, Luis Valledor, Mónica Meijón, Rodrigo Hasbún, Norma Yagüe Yrei, María E. Santamaría, Marta Pérez, Mario Fernádez Fraga, María Berdasco, Roberto Rodríguez Fernández, and María J. Cañal 26. Plant Tissue Culture and Molecular Markers                María Tamayo-Ordoñez, Javier Huijara-Vasconselos, Adriana Quiroz-Moreno, Matilde Ortíz-García, and  Felipe Sánchez-Teyer 27. Biolistic- and Agrobacterium-Mediated Transformation Protocols for Wheat                Cecília Tamáz-Nyitrai, Huw D. Jones, and László Tamás 28. Improved Genetic Transformation of Cork Oak (Quercus suber L.)                Rubén Alvarez-Fernández and Ricardo-Javier Ordás 29. Organelle Transformation                Anjanabha Bhattacharya, Anish Kumar, Nirali Desai, and Seema Parikh 30. Appendix A - The Components of the Culture Media                Víctor M. Loyola-Vargas 31. Appendix B - Plant Biotechnology and Tissue Culture Resources in the Internet                Víctor M. Loyola-Vargas